Tissue microarray use for immunohistochemical study of ameloblastoma

Background Ameloblastoma is a locally aggressive odontogenic tumor with high rates of recurrence. To better understand the molecular basis of ameloblastoma, tissue microarray (TMA) may represent a useful tool. However, despite TMA has been considered a high‐throughput technique for different human n...

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Bibliographic Details
Published in:Journal of oral pathology & medicine 2016-10, Vol.45 (9), p.704-711
Main Authors: Neves-Silva, Rodrigo, Fonseca, Felipe Paiva, de Jesus, Adriana Souza, Pontes, Hélder Antônio Rebelo, Rocha, André Caroli, Brandão, Thais Bianca, Vargas, Pablo Agustin, Lopes, Márcio Ajudarte, de Almeida, Oslei Paes, Santos-Silva, Alan Roger
Format: Article
Language:English
Subjects:
Adult
ameloblastoma
Ameloblastoma - immunology
Ameloblastoma - pathology
Bcl-2
Biomarkers, Tumor - metabolism
cytokeratin
Dentistry
Female
Humans
Jaw Neoplasms - immunology
Jaw Neoplasms - pathology
Ki67
Male
Middle Aged
Retrospective Studies
Tissue Array Analysis - methods
tissue microarray
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Summary:Background Ameloblastoma is a locally aggressive odontogenic tumor with high rates of recurrence. To better understand the molecular basis of ameloblastoma, tissue microarray (TMA) may represent a useful tool. However, despite TMA has been considered a high‐throughput technique for different human neoplasms, it remains to be validated in the ameloblastoma context. Therefore, the objective of this study was to validate TMA for immunohistochemical study of ameloblastoma, determining its most appropriate design. Methods Forty cases of ameloblastoma were manually distributed in two TMA blocks assembled in triplicate containing 1.0‐ and 2.0‐mm cores (20 cases each). Immunohistochemistry for cytokeratins 14 and 19, and Bcl‐2 and Ki‐67 was performed, and semiquantitative analysis was performed. Results obtained with TMA sections were compared to their corresponding conventional whole‐section slides (CWSS). Results Kappa statistical test demonstrated that both 1.0‐ and 2.0‐mm cores assessed as duplicate or triplicate significantly correlated with CWSS, with higher levels obtained using Ki67 (k = 0.98, 0.97, 0.88, 0.87) and CK19 (k = 0.62, 0.58, 0.85, 0.85). There was no significant difference between 1.0‐ and 2.0‐mm cores, and between duplicate and triplicate values. 1.0‐mm TMA showed a higher index of core loss (33.74% vs. 4.99%). Conclusion Using a manual arrayer, it was demonstrated that 1.0‐mm TMA arranged in duplicate is a valid method for ameloblastoma immunohistochemical study with satisfactory levels of agreement between TMA cylinders and CWSS.
ISSN:0904-2512
1600-0714
DOI:10.1111/jop.12428