Influence of gossypol acetic acid on the growth of human adenoid cystic carcinoma ACC-M cells and the expression of DNA methyltransferase 1

• Published in: Genetics and molecular research 2015-10, Vol.14 (4), p.13456-13466
• Main Authors: Wu, Y, Wei, Y-N, Yue, W-Y, Chen, W-F, Fu, S
• Format: Article
• Language: English
• Subjects: Apoptosis - drug effects; Carcinoma, Adenoid Cystic - genetics; Carcinoma, Adenoid Cystic - metabolism; Cell Line, Tumor; Cell Proliferation - drug effects; DNA (Cytosine-5-)-Methyltransferase 1; DNA (Cytosine-5-)-Methyltransferases - genetics; DNA (Cytosine-5-)-Methyltransferases - metabolism; Flow Cytometry; Gossypol - analogs & derivatives; Gossypol - pharmacology; Humans
• ISSN: 1676-5680; 1676-5680
• DOI: 10.4238/2015.October.28.6

We investigated the effects of gossypol acetic acid (GAA) on the proliferation, apoptosis, and expression of DNA methyltransferase 1 (DNMT1) mRNA in human adenoid cystic carcinoma (ACC-M) cells in vitro. The proliferation and apoptosis of ACC-M cells after treatment with different concentrations of GAA were detected using Cell Counting Kit-8 and flow cytometry, respectively. DNMT1 mRNA expression was measured by real-time fluorescence quantitative polymerase chain reaction. The growth of ACC-M cells was inhibited after treatment with GAA for 24, 48, and 72 h. The apoptotic rates of ACC-M cells after treatment with GAA for 72 h were higher than those of control cells (without treatment) (P < 0.05). DNMT1 mRNA expression in ACC-M after treatment with GAA for 72 h was lower than that in control cells (P < 0.05). GAA had inhibitory effects on the proliferation and induced apoptosis of human ACC-M cells, while GAA also reduced the expression level of DNMT1 mRNA in ACC-M cells.