Spermine cycling in mitochondria is mediated by adenine nucleotide translocase activity: mechanism and pathophysiological implications

Spermine, besides to be transported in mitochondria by an energy dependent electrophoretic mechanism, can be also released by two different mechanisms. The first one is induced in deenergizing conditions by FCCP or antimycin A and it is mediated by an electroneutral exchange spermine protons. The se...

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Bibliographic Details
Published in:Amino acids 2016-10, Vol.48 (10), p.2327-2337
Main Authors: Grancara, Silvia, Dalla Via, Lisa, García-Argáez, Aida Nelly, Ohkubo, Shinji, Pacella, Elena, Manente, Sabrina, Bragadin, Marcantonio, Toninello, Antonio, Agostinelli, Enzo
Format: Article
Language:English
Subjects:
Analytical Chemistry
Animals
Antimycin A - pharmacology
Apoptosis
Biochemical Engineering
Biochemistry
Biological Transport, Active - drug effects
Biomedical and Life Sciences
Carbonyl Cyanide p-Trifluoromethoxyphenylhydrazone - pharmacology
Life Sciences
Male
Mitochondria, Liver - metabolism
Mitochondrial ADP, ATP Translocases - metabolism
Mitochondrial Membranes - metabolism
Mitochondrial Proteins - metabolism
Neurobiology
Original Article
Proteomics
Rats
Rats, Wistar
Spermine - metabolism
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Summary:Spermine, besides to be transported in mitochondria by an energy dependent electrophoretic mechanism, can be also released by two different mechanisms. The first one is induced in deenergizing conditions by FCCP or antimycin A and it is mediated by an electroneutral exchange spermine protons. The second one takes place in energizing conditions during the activity of the adenine nucleotide translocase and is mediated by an electroneutral symport mechanism involving the efflux in co-transport of spermine and phosphate and the exchange of exogenous ADP with endogenous ATP. The triggering of this mechanism permits an alternating cycling of spermine across the mitochondrial membrane, that is spermine is transported or released by energized mitochondria in the absence or presence of ATP synthesis, respectively. The physiological implications of this cycling of spermine are related to the induction or prevention of mitochondrial permeability transition and, consequently, on apoptosis or its prevention.
ISSN:0939-4451
1438-2199
DOI:10.1007/s00726-016-2264-6