Cyclic adenosine monophosphate-protein kinase A signal pathway may be involved in pulmonary aquaporin-5 expression in ischemia/reperfusion rats following deep hypothermia cardiac arrest

• Published in: Genetics and molecular research 2016-02, Vol.15 (1)
• Main Authors: Wu, Z Y, Yao, Y, Hu, R, Dai, F F, Zhang, H, Mao, Z F
• Format: Article
• Language: English
• Subjects: Acute Lung Injury - etiology; Acute Lung Injury - metabolism; Animals; Aquaporin 5 - genetics; Cyclic AMP-Dependent Protein Kinases; Disease Models, Animal; Down-Regulation; Heart Arrest - complications; Hypothermia; Ischemia - complications; Ischemia - etiology; Lung - blood supply; Lung - metabolism; Rats; Rats, Wistar; Reperfusion Injury - etiology; Reperfusion Injury - metabolism; Signal Transduction
• ISSN: 1676-5680; 1676-5680
• DOI: 10.4238/gmr.15017377

We investigated the effects of cyclic adenosine monophosphate-protein kinase A (cAMP-PKA) on the expression of aquaporin 5 (AQP5) in ischemia/reperfusion (I/R) rats following deep hypothermia cardiac arrest. Wistar rats were randomly divided into: a sham control group (subjected to a sham operation); an I/R group (subjected to occlusion of the bronchial arteries and the left inferior pulmonary artery); an H89 group (subjected to occlusion of the bronchial arteries and the left inferior pulmonary vein and artery, and treated with 5 mg/kg H89 for 2 days before the study); and a forskolin group (subjected to occlusion of the bronchial arteries and the left inferior pulmonary vein and artery, and treated with 5 mg/kg forskolin for 2 days before the study). Expression levels of AQP5 mRNA and protein were determined using reverse transcription-polymerase chain reaction and western blotting. Decreased expression of AQP5 was noted in the pulmonary tissues of the I/R group compared with the sham controls. Compared to that in the control group, there was a notable decrease in AQP5 expression in the I/R group. After treating with forskolin, AQP5 expression increased in the forskolin group compared with the I/R group. In the H89 group, AQP5 expression decreased compared with the I/R group. The decreased expression of AQP5 was possibly associated with acute pulmonary injury induced by I/R. The cAMP-PKA signal pathway may be involved in the expression of AQP5 in I/R rats after deep hypothermia cardiac arrest.