In Vitro Differentiation of Human Umbilical Cord Blood CD133 super( +) Cells into Insulin Producing Cells in Co-Culture with Rat Pancreatic Mesenchymal Stem Cells

• Published in: Cell journal (Yakhteh) 2015-07, Vol.17 (2), p.211-211
• Main Authors: Samani, Fazel Sahraneshin, Ebrahimi, Marzieh, Zandieh, Tahereh, Khoshchehreh, Reyhaneh, Eslaminejad, Mohamadreza Baghaban, Aghdami, Nasser, Baharvand, Hossein
• Format: Article
• Language: English
• ISSN: 2228-5806; 2228-5814

Pancreatic stroma plays an important role in the induction of pancreatic cells by the use of close range signaling. In this respect, the authors presume that pancreatic mesenchymal cells (PMC) as a fundamental factor of the stromal niche may have an effective role in differentiation of umbilical cord blood cluster of differentiation 133 super( +) (UCB-CD133 super( +)) cells into newly-formed beta -cells in vitro. This study is an experimental research. The UCB-CD133 super( +) cells were purified by magnetic activated cell sorting (MACS) and differentiated into insulin producing cells (IPC) in co-culture, both directly and indirectly with rat PMCs. Immunocytochemistry and enzyme linked immune sorbent assay (ELISA) were used to determine expression and production of insulin and C-peptide at the protein level. The results demonstrated that UCB-CD133 super( +) differentiated into IPCs. Cells in islet-like clusters with (out) co-cultured with rat pancreatic stromal cells produced insulin and C-peptide and released them into the culture medium at the end of the induction protocol. However they did not respond well to glucose challenges.