Comparing automated vs manual leukocyte differential counts for quantifying the ‘left shift’ in the blood of neonates

Objective: The neutrophil ‘left shift’ can be measured via the immature to total (I/T) neutrophil ratio or the absolute bands per μl using a manual differential count. It can also be measured from an automated differential count by the immature granulocyte percentage (IG%) or the absolute IG per μl....

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Bibliographic Details
Published in:Journal of perinatology 2016-10, Vol.36 (10), p.843-848
Main Authors: MacQueen, B C, Christensen, R D, Yoder, B A, Henry, E, Baer, V L, Bennett, S T, Yaish, H M
Format: Article
Language:English
Subjects:
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692/700/1720
Automation
Blood
Blood tests
Comparative analysis
Female
Granulocytes - cytology
Hematology
Humans
Immunoglobulins
Infant
Infant, Newborn
Infants (Newborn)
Infections
Intervals
Leukocyte Count - methods
Leukocytes
Leukocytes (granulocytic)
Leukocytes (neutrophilic)
Medical examination
Medical laboratories
Medicine
Medicine & Public Health
Musical groups
Neonatal care
Neonates
Neutrophils
Newborn babies
Newborn infants
original-article
Pediatric research
Pediatric Surgery
Pediatrics
Reference Values
Technology application
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Summary:Objective: The neutrophil ‘left shift’ can be measured via the immature to total (I/T) neutrophil ratio or the absolute bands per μl using a manual differential count. It can also be measured from an automated differential count by the immature granulocyte percentage (IG%) or the absolute IG per μl. In neonates, it is unknown if the manual or automated differential count is superior. Study Design: We directly compared complete blood counts (CBCs) with manual and automated differential counts from infants
ISSN:0743-8346
1476-5543
DOI:10.1038/jp.2016.92