Selecting soybean resistant to the cyst nematode Heterodera glycines using simple sequence repeat (microssatellite) markers

The soybean cyst nematode (SCN) is a major cause of soybean yield reduction. The objective of this study was to evaluate the efficiency of marker-assisted selection to identify genotypes resistant to SCN race 3 infection, using Sat_168 and Sat-141 resistance quantitative trait loci. The experiment w...

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Bibliographic Details
Published in:Genetics and molecular research 2016-03, Vol.15 (1), p.15016850-15016850
Main Authors: Espindola, S M C G, Hamawaki, O T, Oliveira, A P, Hamawaki, C D L, Hamawaki, R L, Takahashi, L M
Format: Article
Language:English
Subjects:
Animals
Disease Resistance
Genes, Plant
Genetic Markers - genetics
Genetic Variation
Glycine max - genetics
Glycine max - immunology
Glycine max - parasitology
Heterodera glycines
Immunity, Innate
Inbreeding
Microsatellite Repeats
Nematoda
Nematoda - physiology
Plant Diseases - genetics
Plant Diseases - immunology
Quantitative Trait Loci
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Summary:The soybean cyst nematode (SCN) is a major cause of soybean yield reduction. The objective of this study was to evaluate the efficiency of marker-assisted selection to identify genotypes resistant to SCN race 3 infection, using Sat_168 and Sat-141 resistance quantitative trait loci. The experiment was carried out under greenhouse conditions, using soybean populations originated from crosses between susceptible and resistant parent stock: CD-201 (susceptible) and Foster IAC (resistant), Conquista (susceptible) and S83-30 (resistant), La-Suprema (susceptible) and S57-11 (resistant), and Parecis (susceptible) and S65-50 (resistant). Plants were inoculated with SCN and evaluated according to the female index (FI), those with FI < 10% were classified as resistant to nematode infection. Plants were genotyped for SCN resistance using microsatellite markers Sat-141 and Sat_168. Marker selection efficiency was analyzed by a contingency table, taking into account genotypic versus phenotypic evaluations for each line. These markers were shown to be useful tool for selection of SCN race 3.
ISSN:1676-5680
1676-5680
DOI:10.4238/gmr.15016850