Genetic diversity in the germplasm of black pepper determined by EST-SSR markers

This study aimed to assess genetic diversity in the germplasm of black pepper from around the world using SSR markers from EST. In total, 13 markers were selected and successfully amplified the target loci across the black pepper germplasm. All the EST-SSR markers showed high levels of polymorphisms...

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Bibliographic Details
Published in:Genetics and molecular research 2016-03, Vol.15 (1)
Main Authors: Wu, B D, Fan, R, Hu, L S, Wu, H S, Hao, C Y
Format: Article
Language:English
Subjects:
Expressed Sequence Tags
Genetic Speciation
Microsatellite Repeats
Peperomia pellucida
Piper nigrum
Piper nigrum - genetics
Polymorphism, Genetic
Seeds - genetics
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Summary:This study aimed to assess genetic diversity in the germplasm of black pepper from around the world using SSR markers from EST. In total, 13 markers were selected and successfully amplified the target loci across the black pepper germplasm. All the EST-SSR markers showed high levels of polymorphisms with an average polymorphism information content of 0.93. The genetic similarity coefficients among all accessions ranged from 0.724 to 1.000, with an average of 0.867. These results indicated that black pepper germplasms possess a complex genetic background and high genetic diversity. Based on a cluster analysis, 148 black pepper germplasms were grouped in two major clades: the Neotropics and the Asian tropics. Peperomia pellucida was grouped separately and distantly from all other accessions. These results generally agreed with the genetic and geographic distances. However, the Asian tropics clade did not cluster according to their geographic origins. In addition, compared with the American accessions, the Asian wild accessions and cultivated accessions grouped together, indicating a close genetic relationship. This verified the origin of black pepper. The newly developed EST-SSRs are highly valuable resources for the conservation of black pepper germplasm diversity and for black pepper breeding.
ISSN:1676-5680
1676-5680
DOI:10.4238/gmr.15018099