Novel genomic microsatellite markers for genetic population and diversity studies of tropical scalloped spiny lobster (Panulirus homarus) and their potential application in related Panulirus species

Fourteen polymorphic microsatellites with perfect di-, tri-, and tetra-nucleotide repeats were identified for Panulirus homarus using Roche 454 whole-genome sequencing method. Microsatellites were efficiently co-amplified in four multiplexes and a singleplex, providing consistent and easily interpre...

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Bibliographic Details
Published in:Genetics and molecular research 2016-01, Vol.15 (2)
Main Authors: Delghandi, M, Goddard, S, Jerry, D R, Dao, H T, Al Hinai, M S N, Al-Amry, W, Al-Marzouqi, A
Format: Article
Language:English
Subjects:
Animals
Genetic Markers
Genotype
Heterozygote
Linkage Disequilibrium
Microsatellite Repeats
Palinuridae - genetics
Panulirus
Panulirus homarus
Polymorphism, Genetic
Pontophilus spinosus
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Summary:Fourteen polymorphic microsatellites with perfect di-, tri-, and tetra-nucleotide repeats were identified for Panulirus homarus using Roche 454 whole-genome sequencing method. Microsatellites were efficiently co-amplified in four multiplexes and a singleplex, providing consistent and easily interpretable genotypes. The number of alleles per locus ranged from 2 to 11 with the observed and expected heterozygosity ranging between 0.000-0.532 and 0.031-0.836, respectively. A significant deviation from Hardy-Weinberg equilibrium was observed for majority of the loci, probably due to homozygote excess. Genetic linkage disequilibrium analysis between all the possible pairs of the loci showed significant departure from the null hypothesis in the loci pairs Pho-G11-Pho-G33 and Pho-G33-Pho-G57. High success in primer cross-species amplification of these microsatellite markers indicates their utility for genetic studies of different Panulirus species.
ISSN:1676-5680
1676-5680
DOI:10.4238/gmr.15027846