A Microdosimetric Study of Electropulsation on Multiple Realistically Shaped Cells: Effect of Neighbours

Over the past decades, the effects of ultrashort-pulsed electric fields have been used to investigate their action in many medical applications (e.g. cancer, gene electrotransfer, drug delivery, electrofusion). Promising aspects of these pulses has led to several in vitro and in vivo experiments to...

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Bibliographic Details
Published in:The Journal of membrane biology 2016-10, Vol.249 (5), p.691-701
Main Authors: Denzi, Agnese, Camera, Francesca, Merla, Caterina, Benassi, Barbara, Consales, Claudia, Paffi, Alessandra, Apollonio, Francesca, Liberti, Micaela
Format: Article
Language:English
Subjects:
Biochemistry
Biomedical and Life Sciences
Cell Line, Tumor
Cell Membrane
Cell Shape
Cells
Electric fields
Electroporation - methods
Human Physiology
Humans
Life Sciences
Models, Biological
Models, Theoretical
Pulsars
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Summary:Over the past decades, the effects of ultrashort-pulsed electric fields have been used to investigate their action in many medical applications (e.g. cancer, gene electrotransfer, drug delivery, electrofusion). Promising aspects of these pulses has led to several in vitro and in vivo experiments to clarify their action. Since the basic mechanisms of these pulses have not yet been fully clarified, scientific interest has focused on the development of numerical models at different levels of complexity: atomic (molecular dynamic simulations), microscopic (microdosimetry) and macroscopic (dosimetry). The aim of this work is to demonstrate that, in order to predict results at the cellular level, an accurate microdosimetry model is needed using a realistic cell shape, and with their position and packaging (cell density) characterised inside the medium.
ISSN:0022-2631
1432-1424
DOI:10.1007/s00232-016-9912-3