Quantitative detection of viable helminth ova from raw wastewater, human feces, and environmental soil samples using novel PMA-qPCR methods

In this study, we have evaluated the efficacy of propidium monoazide quantitative polymerase chain reaction (PMA-qPCR) to differentiate between viable and non-viable Ancylostoma caninum ova. The newly developed method was validated using raw wastewater seeded with known numbers of A. caninum ova. Re...

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Bibliographic Details
Published in:Environmental science and pollution research international 2016-09, Vol.23 (18), p.18639-18648
Main Authors: Gyawali, P., Ahmed, W., Sidhu, J. P. S., Nery, S. V., Clements, A. C., Traub, R., McCarthy, J. S., Llewellyn, S., Jagals, P., Toze, S.
Format: Article
Language:English
Subjects:
Ancylostoma caninum
Animals
Aquatic Pollution
Ascaris
Atmospheric Protection/Air Quality Control/Air Pollution
Azides
Earth and Environmental Science
Ecotoxicology
Environment
Environmental Chemistry
Environmental Health
Environmental Monitoring - methods
Environmental science
Ethics
Feces
Feces - parasitology
Health risks
Helminths - physiology
Humans
Infections
Methods
Necator americanus
Ovum
Parasites
Propidium - analogs & derivatives
Public health
Raw wastewater
Real-Time Polymerase Chain Reaction - methods
Research Article
Risk assessment
Sanitation
Soil - parasitology
Soils
Studies
Waste Water - parasitology
Waste Water Technology
Wastewater treatment plants
Water Management
Water Pollution Control
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Summary:In this study, we have evaluated the efficacy of propidium monoazide quantitative polymerase chain reaction (PMA-qPCR) to differentiate between viable and non-viable Ancylostoma caninum ova. The newly developed method was validated using raw wastewater seeded with known numbers of A. caninum ova. Results of this study confirmed that PMA-qPCR has resulted in average of 88 % reduction ( P  
ISSN:0944-1344
1614-7499
DOI:10.1007/s11356-016-7039-9