Computerized automated morphometric assay including frequency estimation of pentachlorophenol induced nuclear anomalies (micronucleus) in catfish Heteropneustes fossilis

An in vivo study of the effects of pentachlorophenol was carried out with a pre-acclimatized fish species, Heteropneustes fossilis, using four sub-lethal concentrations, 0.1, 0.2, 0.3 and 0.4 ppm, and three sampling times, 48, 72 and 96 h. Cytogenetic preparations were stained by the haematoxylin-eo...

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Bibliographic Details
Published in:Chromosoma 2002-02, Vol.110 (8), p.570-574
Main Authors: Ahmad, Waseem, Ali, M Niamat, Farah, M Abul, Ateeq, Bushra
Format: Article
Language:English
Subjects:
Animals
Catfishes - genetics
Catfishes - metabolism
Computer Simulation
Erythrocytes - drug effects
Fish
Heteropneustes fossilis
Micronuclei, Chromosome-Defective - drug effects
Micronuclei, Chromosome-Defective - genetics
Micronucleus Tests
pentachlorophenol
Pentachlorophenol - metabolism
Pentachlorophenol - toxicity
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Summary:An in vivo study of the effects of pentachlorophenol was carried out with a pre-acclimatized fish species, Heteropneustes fossilis, using four sub-lethal concentrations, 0.1, 0.2, 0.3 and 0.4 ppm, and three sampling times, 48, 72 and 96 h. Cytogenetic preparations were stained by the haematoxylin-eosin technique. The incidence of micronuclei was scored by a manual and an automated method. Small-sized micronuclei appeared in the cytoplasm in addition to the main nucleus. The frequency of micronucleated erythrocytes peaked at 4 days (96 h) exposure. The percentage ot single micronuclei increased with longer exposures. The Mann-Whitney U test showed all micronuclei frequencies were significantly different from control (P
ISSN:0009-5915
1432-0886
DOI:10.1007/s00412-001-0171-3