TOMATO BUSHY STUNT VIRUS IN NICOTIANA GLAUCA IN GREECE

During the last years, a number of the widely spread weed Nicotiana glauca plants showing virus-like symptoms, were observed in different regions of Greece (Epyrus, Attica and Dodecannesus). From infected plants, showing chlorotic spots, deformation of the leaves and enations, a virus was consistent...

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Bibliographic Details
Published in:Journal of plant pathology 2001-11, Vol.83 (3), p.225-225
Main Authors: Grieco, F., Vovlas, C.
Format: Article
Language:English
Subjects:
Disease Note
Nicotiana benthamiana
Nicotiana glauca
Tomato bushy stunt virus
Tombusvirus
Viruses
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Summary:During the last years, a number of the widely spread weed Nicotiana glauca plants showing virus-like symptoms, were observed in different regions of Greece (Epyrus, Attica and Dodecannesus). From infected plants, showing chlorotic spots, deformation of the leaves and enations, a virus was consistently transmitted by sap inoculation to herbaceous hosts. The results of biological and serological assays as well as the outward aspect of virus particles from symptomatic leaves of artificially infected Nicotiana benthamiana, suggested that the virus could be an isolate of Tomato bushy stunt virus (TBSV; Genus Tombusvirus, Family Tombusviridae) (Russo et al., 1994). RNA extracted from purified virus preparations were subjected to RT-PCR using specific primers [F (+): 5' TGACGCTCATGAGCCCAGCATCC 3'; R (-): 5' TCCTGATCCTCCATCCCAACGTTC 3'] designed on tombusviruses sequences from EMBL database. The amplification product (1368 nt) was cloned and sequenced (Ace. no. AJ312281). This sequence contained the complete coat protein (CP) gene and the amino acid sequence was 91% identical to the CP of TBSV cherry strain (Acc. no. M31019). TBSV is the cause of severe epidemics in several horticoltural crops (Luis-Arteaga et al., 1996). This is the first report of TBSV in N. glauca, which appears to be a new host for this virus.
ISSN:1125-4653
2239-7264