Induction of microspore embryos in a CMS line of Brassica juncea and formation of the androgenic plantlets

• Published in: Euphytica 2001-01, Vol.120 (2), p.195-203
• Main Authors: MEGHNA RANA MALIK, RANGASWAMY, N. S, SHIVANNA, K. R
• Format: Article
• Language: English
• Subjects: Agronomy. Soil science and plant productions; Androgenesis; Anther and ovule culture, utilization for haploid production; Anthers; Biological and medical sciences; Biotechnology; Brassica; Brassica campestris; Brassica juncea; Cell culture; Diplotaxis berthautii; Diplotaxis erucoides; Embryos; Eukaryotic cell cultures; Flowers & plants; Fundamental and applied biological sciences. Psychology; Genetics and breeding of economic plants; Genotypes; Haploidy, in vitro culture applications, somatic hybrids; Hybridization; Hybrids; Methods. Procedures. Technologies; Plant breeding: fundamental aspects and methodology; Plant cells and fungal cells; Plantlets; Pollen; Silver nitrate
• ISSN: 0014-2336; 1573-5060
• DOI: 10.1023/A:1017502113066

Anther culture of two wide hybrids (Diplotaxis erucoides × Brassica campestris) × B. juncea and (D. berthautii × B. campestris) × B. juncea, their CMS lines and the parent species elicited a range of responses highlighting the importance of the genotype. Androgenesis was expressed in cultured anthers of CMS (D. erucoides) B. juncea (22.8%), in restored pollen fertile plants of this CMS line (1.66%), and in the parent, B. juncea cv Pusa Bold (13.02%). AgNO3 was essential for androgenic response in the CMS lines, and it markedly increased the frequency of androgenesis in the cultivated species. Multiple crops of microspore embryos were obtained from responsive anthers of CMS plants in anther recultures. As high as92% microspore embryos of the CMS line germinated on basal B5medium and formed normal plantlets.