Expression of nucleolus, endosperm storage proteins and disease resistance in an amphiploid between Aegilops tauschii and Secale silvestre

• Published in: Euphytica 2001-01, Vol.119 (3), p.317-321
• Main Authors: ZU-JUN, Yang, GUANG-RONG, Li, HUA-REN, Jiang, ZHENG-LONG, Ren
• Format: Article
• Language: English
• Subjects: Aegilops tauschii; Agronomy. Soil science and plant productions; Airborne microorganisms; Biological and medical sciences; chromosome 5; Cytology; Disease resistance; Fundamental and applied biological sciences. Psychology; Genetics; Genetics and breeding of economic plants; Interspecific and intergeneric hybridization, introgressions; Plant breeding: fundamental aspects and methodology; Secale sylvestre
• ISSN: 0014-2336; 1573-5060
• DOI: 10.1023/A:1017591519520

Cytology and gene expression of an amphiploid between Aegilops tauschiiL., native to China, and Secale silvestre L. were studied to reveal the genomic interaction between the donor species. High frequencies of aneuploids were observed in the progenies of the amphiploid, indicating its cytological instability. Feulgen staining and Giemsa-C banding showed that only the nucleolar organizing region from chromosome 5D of Ae. tauschii existed in the amphiploid (2n = 28). The nucleolus of S. silvestre was not observed. Endosperm storage protein electrophoresis indicated most gliadin and glutenin genes from both parents were expressed in the endosperm of the amphiploid. When inoculated by wheat stripe rust and powdery mildew isolates,the amphiploid did not express the resistance from its Secale parent,suggesting the presence of disease resistance suppressor(s) in the D genome of Ae. tauschii as well as nucleolar organizer suppressors.[PUBLICATION ABSTRACT]