Participation of ABH Glycoconjugates in the Secretory Response to Escherichia coli Heat-Labile Toxin in Rabbit Intestine

The ability of membrane ABH blood group-active glycoconjugates to act as receptors of the heat-labile enterotoxin of Escherichia coli (LTh) was studied in vitro and in vivo when GM1 was blocked by the cholera toxin B subunit. Rabbits were classified as AB or H based on intestinal ABH-antigenic activ...

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Bibliographic Details
Published in:The Journal of infectious diseases 1999-08, Vol.180 (2), p.419-425
Main Authors: Galván, Estela M., Roth, German A., Monferran, Clara G.
Format: Article
Language:English
Subjects:
ABO blood group system
ABO Blood-Group System - immunology
Adenylyl Cyclases - metabolism
Animals
Bacterial diseases
Bacterial Toxins - metabolism
Bacterial Toxins - toxicity
Bacteriology
Biological and medical sciences
Cholera
Cholera Toxin - metabolism
Dose-Response Relationship, Drug
Enterotoxins
Enterotoxins - metabolism
Enterotoxins - toxicity
Escherichia coli
Escherichia coli Proteins
Experimental bacterial diseases and models
Fundamental and applied biological sciences. Psychology
G(M1) Ganglioside - metabolism
Glycoconjugates
Glycoconjugates - metabolism
heat-labile enterotoxin
heat-labile toxin
Ileum - metabolism
Infectious diseases
Intestines
Lectins
Lectins - metabolism
Ligands
Major Articles
Medical sciences
Microbiology
Microvilli - metabolism
Pathogenicity, virulence, toxins, bacteriocins, pyrogens, host-bacteria relations, miscellaneous strains
Plant Lectins
Rabbits
Receptors
Secretion
Toxins
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Summary:The ability of membrane ABH blood group-active glycoconjugates to act as receptors of the heat-labile enterotoxin of Escherichia coli (LTh) was studied in vitro and in vivo when GM1 was blocked by the cholera toxin B subunit. Rabbits were classified as AB or H based on intestinal ABH-antigenic activities. Brush border membranes from AB rabbits contained 4 times more LTh binding sites than the H ones. LTh interaction could be inhibited by lectins that recognize ABH determinants. LTh induced a similar dose-dependent secretory response in ligated ileal loops of both types of animals. Anti-AB antibodies and Ulex europaeus I lectin could significantly reduce the fluid accumulation in AB and H rabbits, respectively. LTh caused adenylate cyclase activation even when GM1 was blocked, and this effect was abolished by the addition of specific ABH ligands. These results suggest that ABH glycoconjugates are involved in the host secretory response to LTh in rabbit intestine.
ISSN:0022-1899
1537-6613
DOI:10.1086/314878