A simple and rapid method for analyzing the Monascus pigment-mediated degradation of mutagenic 3-hydroxyamino-1-methyl-5H-pyrido[4,3-b]indole by in-capillary micellar electrokinetic chromatography

A simple and rapid method is described for analyzing the Monascus pigment-mediated degradation of 3-hydroxyamino-1-methyl-5H-pyrido[4,3-b]indole (Trp-P-2(NHOH)). We used the in-capillary micellar electrokinetic chromatography (MEKC). During the electrophoresis, the mutagen and the pigment, due to th...

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Bibliographic Details
Published in:Mutation research. Genetic toxicology and environmental mutagenesis 1999-07, Vol.444 (1), p.75-83
Main Authors: WATANABE, T, TAPAN KUMAR MAZUMDER, YAMAMOTO, A, NAGAI, S, ARIMOTO-KOBAYASHI, S, HAYATSU, H, TERABE, S
Format: Article
Language:English
Subjects:
Biological and medical sciences
Food toxicology
Medical sciences
Monascus
Toxicology
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Summary:A simple and rapid method is described for analyzing the Monascus pigment-mediated degradation of 3-hydroxyamino-1-methyl-5H-pyrido[4,3-b]indole (Trp-P-2(NHOH)). We used the in-capillary micellar electrokinetic chromatography (MEKC). During the electrophoresis, the mutagen and the pigment, due to their different migration velocities, mix for a certain period of time to interact, and then they are separated and quantified. Using this technique, we were able to demonstrate that Trp-P-2(NHOH) is degraded by the pigment. The degradation was pigment-dose dependent, and because the pigment was recovered unchanged, it was deduced that the pigment acted catalytically for the degradation. The entire MEKC procedure takes 8 min.
ISSN:1383-5718
1879-3592
DOI:10.1016/S1383-5718(99)00096-0