The sae locus of Staphylococcus aureus encodes a two-component regulatory system

Abstract sae is a regulatory locus that activates the production of several exoproteins in Staphylococcus aureus. A 3.4-kb fragment of a S. aureus genomic library, screened with a probe adjacent to the transposon insertion of a sae::Tn551 mutant, was cloned into a bifunctional vector. This fragment...

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Bibliographic Details
Published in:FEMS microbiology letters 1999-08, Vol.177 (1), p.15-22
Main Authors: Giraudo, Ana T., Calzolari, Aldo, Cataldi, Angel A., Bogni, Cristina, Nagel, Rosa
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Bacterial Proteins - biosynthesis
Bacterial Proteins - chemistry
Bacterial Proteins - genetics
Bacteriology
Base Sequence
Biological and medical sciences
Chromosome Mapping
Chromosomes
Chromosomes, Bacterial - genetics
Cloning, Molecular
Conserved Sequence
Electrophoresis
Exoprotein synthesis
Fundamental and applied biological sciences. Psychology
Gene Expression Regulation, Bacterial
Genes, Regulator
Genetics
Genomic Library
Global regulatory locus
Histidine
Homology
Kinases
Loci
Microbiology
Molecular Sequence Data
Mutagenesis, Insertional
Protein kinase
Protein Kinases - biosynthesis
Protein Kinases - chemistry
Protein Kinases - genetics
Restoration
Restriction Mapping
Sae cloning
sae gene
saeS gene
Sequence Alignment
Sequence Homology, Amino Acid
Staphylococcus aureus
Staphylococcus aureus - genetics
Staphylococcus aureus - metabolism
Two‐component regulatory system
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Summary:Abstract sae is a regulatory locus that activates the production of several exoproteins in Staphylococcus aureus. A 3.4-kb fragment of a S. aureus genomic library, screened with a probe adjacent to the transposon insertion of a sae::Tn551 mutant, was cloned into a bifunctional vector. This fragment was shown to carry the sae locus by restoration of exoprotein production in sae mutants. The sae locus was mapped to the SmaI-D fragment of the staphylococcal chromosome by pulse-field electrophoresis. Sequence analysis of the cloned fragment revealed the presence of two genes, designated saeR and saeS, encoding a response regulator and a histidine protein kinase, respectively, with high homology to other bacterial two-component regulatory systems.
ISSN:0378-1097
1574-6968
DOI:10.1111/j.1574-6968.1999.tb13707.x