Amino Acid Sequence of Amylase Type Alpha, MiAmy, from Ok-Rong Mango (Mangifera indica Linn. cv. Ok-Rong)

The 594-amino acid residue sequence of alpha -amylase, MiAmy, from the Ok-rong mango (Mangifera indica Linn. cv. Ok-rong), in the ripening stage, was determined through Reverse Transcription Polymerase Chain Reaction (RT-PCR). Sequence alignments and evolutionary tree analyses revealed its high simi...

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Bibliographic Details
Published in:American journal of biochemistry and biotechnology 2015, Vol.11 (3), p.119-126
Main Authors: Ubonbal, Raksmont, Posoongnoen, Saijai, Daduang, Jureerut, Klaynongsruang, Sompong, Daduang, Sakda
Format: Article
Language:English
Subjects:
Mangifera indica
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Summary:The 594-amino acid residue sequence of alpha -amylase, MiAmy, from the Ok-rong mango (Mangifera indica Linn. cv. Ok-rong), in the ripening stage, was determined through Reverse Transcription Polymerase Chain Reaction (RT-PCR). Sequence alignments and evolutionary tree analyses revealed its high similarity to plastid alpha -amylase from many other plants. The sequence was revealed to have four conserved regions with catalytic amino acid residues for the active site. It was classified as a member of alpha -amylase family 13 because it has an active Domain A, similar to a ( beta / alpha ) sub( 8)-barrel structure. Three-dimensional structural predictions revealed that this partial sequence completely covered all of necessary domains for amylase activity.
ISSN:1553-3468
1558-6332
DOI:10.3844/ajbbsp.2015.119.126