Expression of Plasmodium falciparum C-terminal region of merozoite surface protein (PfMSP1 19), a potential malaria vaccine candidate, in tobacco

A gene encoding the C-terminal region of a major surface antigen of Plasmodium falciparum referred as PfMSP1 19, a leading vaccine candidate for malaria, was cloned in plant transformation vector pBI121. Tobacco leaves were transformed by Agrobacterium tumefaciens carrying the vector and kanamycin r...

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Published in:Plant science (Limerick) 2002-03, Vol.162 (3), p.335-343
Main Authors: Ghosh, Sagar, Malhotra, Pawan, Lalitha, P.V, Guha-Mukherjee, Sipra, Chauhan, V.S
Format: Article
Language:English
Subjects:
Agrobacterium
Biological and medical sciences
Biotechnology
Fundamental and applied biological sciences. Psychology
Genetic engineering
Genetic technics
Malaria vaccine candidate
Merozoite surface antigen
merozoite surface protein
Methods. Procedures. Technologies
Nicotiana tabacum
PfMSP1 19
Plant produced vaccine
Plasmodium falciparum
Protozoa
Transgenic animals and transgenic plants
Transgenic plants
Tropical medicine
Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies
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Summary:A gene encoding the C-terminal region of a major surface antigen of Plasmodium falciparum referred as PfMSP1 19, a leading vaccine candidate for malaria, was cloned in plant transformation vector pBI121. Tobacco leaves were transformed by Agrobacterium tumefaciens carrying the vector and kanamycin resistant plants were regenerated. The Pfmsp1 19 gene, in the transformed plants was identified by polymerase chain reaction amplification, Southern and northern hybridisation techniques. Expression of PfMSP1 19 protein was analysed by enzyme linked immunosorbent assay (ELISA) and Immunoblot assay, which indicated that transformed plants expressed MSP1 19 and displayed structural and immunological characteristics identical to the Escherichia coli expressed protein. This result is a step forward towards the development of an edible and low cost subunit vaccine against malaria, which is a fatal disease of the developing countries.
ISSN:0168-9452
1873-2259
DOI:10.1016/S0168-9452(01)00555-6