A gene encoding Achlya bisexualis β-amylase and its expression in Saccharomyces cerevisiae

Part of a beta -amylase genomic DNA sequence from the oomycete, Achlya bisexualis was cloned by polymerase chain reaction (PCR) using degenerate oligonucleotide primers derived from the conserved regions of other known beta -amylase sequences. The 5'- and 3'-regions of the beta -amylase ge...

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Published in:Biotechnology letters 2001-07, Vol.23 (14), p.1101-1107
Main Authors: KIM, Hee-Ok, PARK, Jeong-Nam, SHIN, Dong-Jun, LEE, Hwanghee Blaise, CHUN, Soon-Bai, SUK BAI
Format: Article
Language:English
Subjects:
Achlya bisexualis
Biological and medical sciences
Biology of microorganisms of confirmed or potential industrial interest
Biotechnology
DNA sequencing
Fundamental and applied biological sciences. Psychology
Miscellaneous
Mission oriented research
Saccharomyces cerevisiae
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container_end_page 1107
container_issue 14
container_start_page 1101
container_title Biotechnology letters
container_volume 23
creator KIM, Hee-Ok
PARK, Jeong-Nam
SHIN, Dong-Jun
LEE, Hwanghee Blaise
CHUN, Soon-Bai
SUK BAI
description Part of a beta -amylase genomic DNA sequence from the oomycete, Achlya bisexualis was cloned by polymerase chain reaction (PCR) using degenerate oligonucleotide primers derived from the conserved regions of other known beta -amylase sequences. The 5'- and 3'-regions of the beta -amylase gene were amplified by genome walking method. The Ach. bisexualis beta -amylase gene consisted of a 1338 bp open reading frame, encoding a protein of 446 amino acids with a molecular weight of 49 381 Da, and was not interrupted by any intron. The deduced amino acid sequence of the beta -amylase gene had 67% similarity to the beta -amylase of Saprolegnia ferax, followed by 40% similarity to that of Arabidopsis thaliana. The beta -amylase gene was expressed in Saccharomyces cerevisiae placing it under the control of the alcohol dehydrogenase gene (ADC1) promoter.
doi_str_mv 10.1023/A:1010503731486
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subjects Achlya bisexualis
Biological and medical sciences
Biology of microorganisms of confirmed or potential industrial interest
Biotechnology
DNA sequencing
Fundamental and applied biological sciences. Psychology
Miscellaneous
Mission oriented research
Saccharomyces cerevisiae
title A gene encoding Achlya bisexualis β-amylase and its expression in Saccharomyces cerevisiae
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