Post-transcriptional Regulation of Luteinizing Hormone Receptor mRNA in the Ovary by a Novel mRNA-binding Protein

Luteinizing hormone (LH) receptor mRNA is post-transcriptionally regulated. An ovarian cytosolic LH receptor mRNA-binding protein (LRBP) identified in our laboratory binds to a polypyrimidine-rich bipartite sequence in the coding region of LH receptor mRNA. The present studies show a role for LRBP i...

Full description

Saved in:
Bibliographic Details
Published in:The Journal of biological chemistry 2002-06, Vol.277 (24), p.21468-21473
Main Authors: Nair, Anil K, Kash, John C, Peegel, Helle, Menon, K M J
Format: Article
Language:English
Subjects:
Animals
Blotting, Northern
Cholesterol Side-Chain Cleavage Enzyme - metabolism
Chorionic Gonadotropin - metabolism
DNA, Complementary - metabolism
Dose-Response Relationship, Drug
Down-Regulation
Female
LH receptor mRNA-binding protein
LRBP protein
Ovary - metabolism
Polyribosomes - metabolism
Protein Structure, Tertiary
Rats
Rats, Sprague-Dawley
Receptors, LH - metabolism
Ribonucleoproteins - chemistry
Ribonucleoproteins - metabolism
RNA - metabolism
RNA Processing, Post-Transcriptional
RNA, Messenger - metabolism
RNA-Binding Proteins - chemistry
RNA-Binding Proteins - metabolism
Time Factors
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Luteinizing hormone (LH) receptor mRNA is post-transcriptionally regulated. An ovarian cytosolic LH receptor mRNA-binding protein (LRBP) identified in our laboratory binds to a polypyrimidine-rich bipartite sequence in the coding region of LH receptor mRNA. The present studies show a role for LRBP in the regulation of LH receptor mRNA. We demonstrated that increased LH receptor mRNA degradation occurs during hormone-induced LH receptor down-regulation. Furthermore, increased degradation of LH receptor mRNA was seen when partially purified LRBP was included in an in vitro mRNA decay reaction. The LH receptor mRNA binding activity of LRBP measured by RNA electrophoretic mobility shift analysis showed an inverse relationship to LH receptor mRNA levels during different physiological states. These results suggest that LRBP is a physiological regulator of LHR mRNA expression in the ovary and provides a novel mechanism for the regulation of LH receptor expression in the ovary.
ISSN:0021-9258
1083-351X
DOI:10.1074/jbc.M111653200