Development of transgenic rice pure lines with enhanced resistance to rice brown planthopper

Mature seed-derived callus from an elite Chinese japonica rice cv. Ewan 5 was cotransformed with two plasmids, pWRG1515 and pRSSGNA1, containing the selectable marker hygromycin phosphotransferase gene (hpt), the reporter beta -glucuronidase gene (gusA) and the snowdrop (Galanthus nivalis) lectin ge...

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Bibliographic Details
Published in:In vitro cellular & developmental biology. Plant 2001-06, Vol.37 (3), p.334-340
Main Authors: Tang, Kexuan, Hu, Quanan, Sun, Xiaofen, Wan, Bingliang, Qi, Huaxiong, Lu, Xinggui
Format: Article
Language:English
Subjects:
Galanthus nivalis
hpt gene
Nilaparvata lugens
Oryza sativa
phosphotransferase gene
usA gene
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Summary:Mature seed-derived callus from an elite Chinese japonica rice cv. Ewan 5 was cotransformed with two plasmids, pWRG1515 and pRSSGNA1, containing the selectable marker hygromycin phosphotransferase gene (hpt), the reporter beta -glucuronidase gene (gusA) and the snowdrop (Galanthus nivalis) lectin gene (gna) via particle bombardment. Thirty-five independent transgenic rice plants were regenerated from 177 bombarded calluses. Eighty-three percent of the transgenic plants contained all three genes, as revealed by Southern blot analysis. Western blot analysis revealed that 23 out of 29 gna-containing transgenic plants expressed Galanthus nivalis agglutinin (GNA) (79%) at various levels with the highest expression being approximately 0.5% of total soluble protein. Genetic analysis confirmed Mendelian segregation of all three transgenes (gna, hpt and gusA) in the R2 progeny. Amongst the R2 generation, two independent homozygous lines were identified that expressed all three transgenes. Insect bioassay and feeding tests showed that these homozygous lines had significant inhibition to rice brown planthopper (Nilaparvata lugens, BPH) by decreasing the survival, overall fecundity of BPH, retarding development, and decreasing the feeding of BPH. These BPH-resistant lines have been incorporated into a rice insect resistance breeding program. This is the first report that homozygous transgenic rice lines expressing GNA, developed by genetic transformation and through genetic analysis-based selection, conferred enhanced resistance to BPH.
ISSN:1054-5476
DOI:10.1079/IVP2000151