Super Temporal-Resolved Microscopy (STReM)

Super-resolution microscopy typically achieves high spatial resolution, but the temporal resolution remains low. We report super temporal-resolved microscopy (STReM) to improve the temporal resolution of 2D super-resolution microscopy by a factor of 20 compared to that of the traditional camera-limi...

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Bibliographic Details
Published in:The journal of physical chemistry letters 2016-11, Vol.7 (22), p.4524-4529
Main Authors: Wang, Wenxiao, Shen, Hao, Shuang, Bo, Hoener, Benjamin S, Tauzin, Lawrence J, Moringo, Nicholas A, Kelly, Kevin F, Landes, Christy F
Format: Article
Language:English
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Summary:Super-resolution microscopy typically achieves high spatial resolution, but the temporal resolution remains low. We report super temporal-resolved microscopy (STReM) to improve the temporal resolution of 2D super-resolution microscopy by a factor of 20 compared to that of the traditional camera-limited frame rate. This is achieved by rotating a phase mask in the Fourier plane during data acquisition and then recovering the temporal information by fitting the point spread function (PSF) orientations. The feasibility of this technique is verified with both simulated and experimental 2D adsorption/desorption and 2D emitter transport. When STReM is applied to measure protein adsorption at a glass surface, previously unseen dynamics are revealed.
ISSN:1948-7185
1948-7185
DOI:10.1021/acs.jpclett.6b02098