Seasonality of freshwater bacterioplankton diversity in two tropical shallow lakes from the Brazilian Atlantic Forest

• Published in: FEMS microbiology ecology 2017-01, Vol.93 (1), p.1
• Main Authors: Ávila, Marcelo P., Staehr, Peter A., Barbosa, Francisco A. R., Chartone-Souza, Edmar, Nascimento, Andréa M. A.
• Format: Article
• Language: English
• Subjects: Actinobacteria - genetics; Actinobacteria - isolation & purification; Bacteria; Bacteria - classification; Bacteria - genetics; Bacteria - isolation & purification; Brazil; Environmental aspects; Forests; Forests and forestry; Genetic aspects; Lakes; Lakes - chemistry; Lakes - microbiology; Phylogeny; Physiological aspects; Plankton - classification; RNA, Bacterial - genetics; RNA, Ribosomal, 16S - genetics; Synechococcus - genetics; Synechococcus - isolation & purification
• ISSN: 1574-6941; 0168-6496; 1574-6941
• DOI: 10.1093/femsec/fiw218

Abstract Bacteria are highly important for the cycling of organic and inorganic matter in freshwater environments; however, little is known about the diversity of bacterioplankton in tropical systems. Studies on carbon and nutrient cycling in tropical lakes suggest a very different seasonality from that of temperate climates. Here, we used 16S rRNA gene next-generation sequencing (NGS) to investigate seasonal changes in bacterioplankton communities of two tropical lakes, which differed in trophic status and mixing regime. Our findings revealed seasonally and depth-wise highly dynamic bacterioplankton communities. Differences in richness and structure appeared strongly related to the physicochemical characteristics of the water column, especially phosphate, pH and oxygen. Bacterioplankton communities were dominated by common taxonomic groups, such as Synechococcus and Actinobacteria acI, as well as rare and poorly characterized taxa such as ‘Candidatus Methylacidiphilum’ (Verrucomicrobia). Stratification and oxygen depletion during the rainy season promoted the occurrence of anoxygenic phototrophic and methanotrophic bacteria important for carbon and nutrient cycling. Differences in lake mixing regime were associated with seasonal beta diversity. Our study is the first attempt to use NGS for cataloging the diversity of bacterioplankton communities in Brazilian lakes and thus contributes to the ongoing worldwide endeavor to characterize freshwater lake bacterioplankton signatures. This study presents the first large 16S rRNA dataset describing bacterioplankton from tropical Brazilian lakes and reveals highly diverse and dynamic communities, helping to map global freshwater lake bacterioplankton.