Polyphenolic-polysaccharide conjugates of Sanguisorba officinalis L. with anticoagulant activity mediated mainly by heparin cofactor II

A macromolecular complex has been isolated from the dried flowering parts of medicinal plant Sanguisorba officinalis L. (So) by multi-step extraction procedure, including that with extraction by organic solvents to degrease the plant material, then with hot alkali, followed by neutralization, partit...

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Bibliographic Details
Published in:International journal of biological macromolecules 2016-12, Vol.93 (Pt A), p.1019-1029
Main Authors: Pawlaczyk-Graja, Izabela, Balicki, Sebastian, Ziewiecki, Rafał, Matulová, Mária, Capek, Peter, Gancarz, Roman
Format: Article
Language:English
Subjects:
Anticoagulant activity
Anticoagulants - isolation & purification
Anticoagulants - pharmacology
Drug Evaluation, Preclinical
Flowers - chemistry
Heparin cofactor II
Heparin Cofactor II - physiology
Humans
NMR
Partial Thromboplastin Time
Plant Extracts - isolation & purification
Plant Extracts - pharmacology
Plant glycoconjugate
Polyphenols - isolation & purification
Polyphenols - pharmacology
Polysaccharides - isolation & purification
Polysaccharides - pharmacology
Prothrombin Time
Sanguisorba - chemistry
Sanguisorba officinalis
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Summary:A macromolecular complex has been isolated from the dried flowering parts of medicinal plant Sanguisorba officinalis L. (So) by multi-step extraction procedure, including that with extraction by organic solvents to degrease the plant material, then with hot alkali, followed by neutralization, partitioning with organic solvents and dialysis. The complex was purified by size-exclusion chromatography into five fractions labeled as So1-So5. Individual fractions differed in the chemical composition and molecular weight distribution patterns. In vitro anticoagulant activity tests showed in all fractions more or less important inhibition of plasma clots, however, So3 and So4 were the most active. The anticoagulant activity of So3 was even more significant than that of the unfractionated complex So. These S. officinalis conjugates were able to inhibit mainly the activity of thrombin when they were mediated by heparin cofactor II, but what was unexpected they were the non-direct inhibitors of factor Xa, mediated by antitrombin, where such mechanism of action is typical for a highly sulphated glycosaminoglycans.
ISSN:0141-8130
1879-0003
DOI:10.1016/j.ijbiomac.2016.09.082