Preparation and characterization of high purity β-chitin from squid pens (Loligo chenisis)

•β-Chitin was successfully prepared from Squid pens Loligo Chenesis.•Products had a high molecular weight with a negligible protein and minerals content.•β-Chitin with long chains and high purity is suitable for producing high quantity β-chitosan. Squid pens were extracted by a NaOH solution at 80°C...

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Bibliographic Details
Published in:International journal of biological macromolecules 2016-12, Vol.93 (Pt A), p.442-447
Main Authors: Cuong, Hoang Ngoc, Minh, Nguyen Cong, Van Hoa, Nguyen, Trung, Trang Si
Format: Article
Language:English
Subjects:
Animals
Chitin - chemistry
Chitin - isolation & purification
Loligo - chemistry
Loligo chenesis
Squid pens
β-Chitin
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Summary:•β-Chitin was successfully prepared from Squid pens Loligo Chenesis.•Products had a high molecular weight with a negligible protein and minerals content.•β-Chitin with long chains and high purity is suitable for producing high quantity β-chitosan. Squid pens were extracted by a NaOH solution at 80°C for 10h to remove protein and minerals. The as-prepared β-chitin had a high molecular weight (8.5±0.1×103kDa), a low protein content (0.63±0.02wt.%), and a negligible amount of minerals. This method avoids the conventional method for the removal of minerals from shrimp and crab shells by HCl. The purity of resulting products was measured by NMR and FTIR. Moreover, the morphology and crystallinity of β-chitin was characterized by SEM and XRD. The β-chitin with long chains and high purity is suitable for producing high quantity β-chitosan for various potential applications.
ISSN:0141-8130
1879-0003
DOI:10.1016/j.ijbiomac.2016.08.085