Bacterial microbiota profile in gills of modified atmosphere-packaged oysters stored at 4 °C

As filter-feeding bivalves, oysters can accumulate microorganisms into their gills, causing spoilage and potential safety issues. This study aims to investigate the changes in the gill microbiota of oysters packed under air and modified atmospheres (MAs, 50% CO2: 50% N2, 70% CO2: 30% O2, and 50% CO2...

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Bibliographic Details
Published in:Food microbiology 2017-02, Vol.61, p.58-65
Main Authors: Chen, Huibin, Wang, Meiying, Lin, Xiangzhi, Shi, Caihua, Liu, Zhiyu
Format: Article
Language:English
Subjects:
Animals
Bacterial Load
Carbon Dioxide
Denaturing Gradient Gel Electrophoresis
DNA, Bacterial - genetics
Food Microbiology
Food Packaging
Genetic Variation
Gill
Gills - microbiology
Microbiota
Microbiota - genetics
Modified atmosphere package
Ostreidae - microbiology
Oxygen
Oyster
PCR-DGGE
Polymerase Chain Reaction
Refrigeration
Seafood - microbiology
Shellfish - microbiology
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Summary:As filter-feeding bivalves, oysters can accumulate microorganisms into their gills, causing spoilage and potential safety issues. This study aims to investigate the changes in the gill microbiota of oysters packed under air and modified atmospheres (MAs, 50% CO2: 50% N2, 70% CO2: 30% O2, and 50% CO2: 50% O2) during storage at 4 °C. The diversity of bacterial microbiota in oyster gills was profiled through polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE) analysis on the 16S rRNA gene V3 region to describe the variation during the entire storage period. The DGGE profile revealed high bacterial diversity in the air- and MA-packaged oyster gills, and the spoilage bacterial microbiota varied in the MA-packaged oyster gills. Results indicated that CO2:O2 (70%:30%) was suitable for oyster MA packaging and that high bacterial loads in oyster gills need to be considered during storage. In addition, Lactobacillus and Lactococcus species were found to grow dominantly in fresh oyster gills under MA packaging, which supports the potential application of MA packaging for oyster storage. •A high diversity of bacteria was observed in air- and MA-packaged oyster gills.•The spoilage bacterial microbiota in oyster gills varied under MA packaging.•CO2:O2 (70%:30%) was more suitable for oyster MA packaging than the other atmospheric conditions.
ISSN:0740-0020
1095-9998
DOI:10.1016/j.fm.2016.08.006