Effects of Humic Acids Isolated from Peat of Various Origin on in Vitro Production of Nitric Oxide: a Screening Study

A screening study of biological activity of native humic acids isolated from peat was performed; several physical and chemical parameters of their structures were studied by UV- and infrared spectroscopy. Spectroscopy yielded similar shape of light absorption curves of humic acids of different origi...

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Bibliographic Details
Published in:Bulletin of experimental biology and medicine 2016-09, Vol.161 (5), p.687-692
Main Authors: Trofimova, E. S., Zykova, M. V., Ligacheva, A. A., Sherstoboev, E. Yu, Zhdanov, V. V., Belousov, M. V., Yusubov, M. S., Krivoshchekov, S. V., Danilets, M. G., Dygai, A. M.
Format: Article
Language:English
Subjects:
Analysis
Animals
Biomedical and Life Sciences
Biomedicine
Cell Biology
Cells, Cultured
Drug Evaluation, Preclinical
Female
Humic acids
Humic Substances
Infrared spectroscopy
Internal Medicine
Laboratory Medicine
Macrophages
Macrophages, Peritoneal - drug effects
Macrophages, Peritoneal - metabolism
Male
Mice, Inbred C57BL
Nitric oxide
Nitric Oxide - biosynthesis
Pathology
Peat
Phosphates
Soil - chemistry
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Summary:A screening study of biological activity of native humic acids isolated from peat was performed; several physical and chemical parameters of their structures were studied by UV- and infrared spectroscopy. Spectroscopy yielded similar shape of light absorption curves of humic acids of different origin, which can reflect similarity of general structural principles of these substances. Alkaline humic acids have more developed system of polyconjugation, while molecular structures of pyrophosphate humic acids were characterized by higher aromaticity and condensation indexes. Biological activity of the studied humic acids was assessed by NO-stimulating capacity during their culturing with murine peritoneal macrophages in a wide concentration range. It was shown that due to dose-dependent enhancement of NO production humic acids can change the functional state of macrophages towards development of pro-inflammatory properties. These changes were associated with high activity of humic acids isolated by pyrophosphate extraction, which allows considering effects of isolation method on biological activity.
ISSN:0007-4888
1573-8221
DOI:10.1007/s10517-016-3486-z