Encapsulation of Biological and Chemical Agents for Plant Nutrition and Protection: Chitosan/Alginate Microcapsules Loaded with Copper Cations and Trichoderma viride

• Published in: Journal of agricultural and food chemistry 2016-11, Vol.64 (43), p.8073-8083
• Main Authors: Vinceković, Marko, Jalšenjak, Nenad, Topolovec-Pintarić, Snježana, Đermić, Edyta, Bujan, Marija, Jurić, Slaven
• Format: Article
• Language: English
• Subjects: Alginates - chemistry; Biological Control Agents - chemistry; Capsules - chemistry; Cations; Chitosan - chemistry; Copper - chemistry; Copper - pharmacology; Fertilizers - microbiology; Glucuronic Acid - chemistry; Hexuronic Acids - chemistry; Spores, Fungal - drug effects; Trichoderma - drug effects
• ISSN: 0021-8561; 1520-5118
• DOI: 10.1021/acs.jafc.6b02879

Novel chitosan/alginate microcapsules simultaneously loaded with copper cations and Trichoderma viride have been prepared and characterized. Information about the intermolecular interactions between biopolymers and bioactive agents was obtained by Fourier transform infrared spectroscopy. Encapsulation of T. viride spores and the presence of copper cations in the same compartment does not inhibit their activity. Microcapsule loading capacity and efficiency as well as swelling behavior and release depend on both the size of the microcapsule and bioactive agents. The in vitro copper cation release profile was fitted to a Korsmeyer–Peppas empirical model. Fickian diffusion was found to be a rate-controlling mechanism of release from smaller microcapsules, whereas anomalous transport kinetics controlled release from larger microcapsules. The T. viride spore release profile exhibited exponential release over the initial lag time. The results obtained opened perspectives for the future use of chitosan/alginate microcapsules simultaneously loaded with biological and chemical agents in plant nutrition and protection.