Loading…
Evaluation of Calcium Dihydroxide- and Silver-Coated Implants in the Rat Tibia
Background Silver ions (Ag+) have strong antibacterial effects, and silver-coated materials are in widespread clinical use. However, the application of silver-coated medical devices is not without concerns: its use with direct bone contact is not established, and systemic toxic side effects of relea...
Saved in:
Published in: | Journal of applied biomaterials & functional materials 2016-11, Vol.14 (4), p.441-448 |
---|---|
Main Authors: | , , , , , , , , , , , |
Format: | Article |
Language: | English |
Subjects: | |
Citations: | Items that this one cites Items that cite this one |
Online Access: | Request full text |
Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
Summary: | Background
Silver ions (Ag+) have strong antibacterial effects, and
silver-coated materials are in widespread clinical use. However, the
application of silver-coated medical devices is not without concerns: its
use with direct bone contact is not established, and systemic toxic side
effects of released Ag+ have been described. Therefore,
alternative bactericidal coatings with a more localized way of acting –
e.g., calcium dihydroxide, Ca(OH)2 (CH) – would be
advantageous.
Methods
A new rat model of the animal's tibial metaphysis was developed. In the left
proximal tibiae of 36 male Wistar rats, titanium screws were implanted. The
screws were coated with hydroxyapatite (HA; 12 animals: group I), low-dosed
HA silver (HA-Ag; 12 animals: group II) and CH (12 animals: group III).
After 6 weeks, all rats were sacrificed. The implants were evaluated for
morphological changes on their surfaces, by light microscopy, scanning
electron microscopy and energy-dispersive X-ray spectroscopy; for
osteointegration, by measurement of resistance to removal; and for bacterial
colonization, by quantitative culture analysis. Additionally, the tibial
bone was investigated histologically for signs of osteomyelitis and
sonicated to detect bacterial loads.
Results
(i) No microbiological or histological signs of infection could be determined
on any of the screws or the surrounding bone. (ii) The bone-implant
interface analysis revealed extensive bone formation and direct bone-implant
contact on all HA, HA-Ag and HA-CH coated screws. (iii) HA and HA-Ag were
partially, and CH was fully, degraded on the screw coating, allowing host
bone to osteointegrate. |
---|---|
ISSN: | 2280-8000 2280-8000 |
DOI: | 10.5301/jabfm.5000323 |