First study about the development of adult Echinococcus canadensis G6 genotype of goat origin in experimentally infected dogs

• Published in: Veterinary parasitology 2016-09, Vol.228, p.6-12
• Main Authors: Soriano, Silvia Viviana, Debiaggi, María Florencia, Pierangeli, Nora Beatriz, Pianciola, Luis Alfredo, Bergagna, Héctor Fabián Jesús, Lazzarini, Lorena Evelina, Mazzeo, Melina Leonor, Basualdo, Juan Angel
• Format: Article
• Language: English
• Subjects: Animals; Disease Models, Animal; Dogs; Echinococcosis - parasitology; Echinococcosis - veterinary; Echinococcus - genetics; Echinococcus - growth & development; Echinococcus - ultrastructure; Echinococcus canadensis G6 genotype; Echinococcus granulosus - genetics; Echinococcus granulosus - growth & development; Echinococcus granulosus - ultrastructure; Echinococcus granulosus sensu lato; Female; Genotype; Goat Diseases - parasitology; Goats; Hook morphometry; Male; Parasite development; Prepatent period
• ISSN: 0304-4017; 1873-2550
• DOI: 10.1016/j.vetpar.2016.08.008

[Display omitted] •Development of E. canadensis G6 of goat origin in experimentally infected dogs.•The prepatent period of the strobilar stage could be genetically determined by the E. granulosus sensu lato genotype.•Prepatent period of the strobilar stage of E. canadensis G6: at least 41days.•Growth rate of E. canadensis G6 intestinal stages of goat origin: about 80μm/day. Echinococcus granulosus sensu lato (E. granulosus sl) must be considered as a species complex, comprising Echinococcus granulosus sensu stricto (E. granulosus ss, genotypes G1-G3), Echinococcus equinus (G4), Echinococcus ortleppi (G5) and Echinococcus canadensis (G6-G10) although the species status of E. canadensis is still controversial. These genotypes closely match the intermediate hosts associated strains described in earlier times among which E. canadensis G6 corresponds to the camel strain. As there are no studies concerning the development of adult stages of the G6 genotype from non-camel origin, the aims of the present study were: to characterize for the first time the development of E. canadensis G6 in dogs experimentally infected with protoscoleces derived from goats, to describe the resultant adult morphology, to evaluate the growth of their rostellar hooks from larval to adult stages and to determine the prepatent period of the strobilar stage of E. canadensis G6 derived from goats. The development of the strobilar stage of E. canadensis G6 genotype of goat origin was examined by studying the growth (variation of the total worm length) and segmentation in experimentally infected dogs at 14, 25, 35 and 56days post infection. A morphological characterization of 35-day-old worms as well as of larval and adult rostellar hooks was also carried out by conventional optical microscopic observations and/or by scanning electron microscopy. The prepatent period of the strobilar stage was assessed by microscopic examination of faeces from 2 infected dogs. Our results were compared with published data from the camel and other strains. The roles of the host, genotype and species in morphological and developmental features as well as the taxonomic position of E. canadensis G6 were discussed. The prepatent period of E. canadensis G6 genotype of goat origin was determined as at least, 41days. The obtained results contribute to increase the knowledge about the biology and genetics of E. granulosus sl complex and are also of practical usefulness for the design of disease control strategies.