Extraction‐spectrophotometric determination of tris(2‐chloroethyl)amine using phthaleins

• Published in: Drug testing and analysis 2017-06, Vol.9 (6), p.916-923
• Main Authors: Rozsypal, Tomas, Halamek, Emil
• Format: Article
• Language: English
• Subjects: Alkylating Agents - analysis; Alkylating Agents - isolation & purification; Buffers; Chemical Warfare Agents - analysis; Chemical Warfare Agents - isolation & purification; Drug testing; Hydrogen-Ion Concentration; Limit of Detection; nitrogen mustard; Nitrogen Mustard Compounds - analysis; Nitrogen Mustard Compounds - isolation & purification; Phenolphthaleins - chemistry; phthalein; spectrophotometry; Spectrophotometry - methods; tris(2‐chloroethyl)amine; Water - analysis
• ISSN: 1942-7603; 1942-7611
• DOI: 10.1002/dta.2094

Procedures for the extraction‐spectrophotometric determination of tris(2‐chloroethyl)amine, an alkylating agent known as a drug as well as a chemical warfare agent (nitrogen mustard HN‐3), with 7 acid‐base indicators of a triphenylmethane lactone type, phthaleins, were developed. Representatives of phthaleins without an oxygen bridge (thymolphthalein, o‐cresolphthalein, naphtholphthalein) and with an oxygen bridge (fluorescein, 2',7'‐dichlorofluorescein, eosin B and eosin Y) were used. The methods were based on the formation of ion pair complexes. Chloroform was used as a non‐polar solvent for an extraction. The conditions to determine were optimized for the optimal pH of the buffer and the concentration of a phthalein as a reagent. The dependence on the reaction time in a water phase and the stoichiometry of extraction products were studied. The detection limits and the limits of the determination of separate procedures and conditional extraction constants were determined. Comparison with the spectrophotometric method of the group determination of alkyl halides and acyl halides using alkaline ethanol‐water solution of thymolphthalein, the so‐called T‐135 agent, was conducted. While studying the selectivity, the possible interference of bis(2‐chloroethyl)sulphide and 3 nitrogen mustards in the proposed procedures were verified. Copyright © 2016 John Wiley & Sons, Ltd. The procedures of the extraction‐spectrophotometric determination of tris (2‐chloroethy)amine, alkylating agent known as a drug as well as a chemical warfare agentnitrogen mustard HN‐3, with 7 acid‐base indicators of a triphenylmethane lactone type, phthaleins, are presented.