Adhesion of Blood Plasma Proteins and Platelet-rich Plasma on l ‑Valine-Based Poly(ester urea)

The competitive absorption of blood plasma components including fibrinogen (FG), bovine serum albumin (BSA), and platelet-rich plasma (PRP) on l -valine-based poly­(ester urea) (PEU) surfaces were investigated. Using four different PEU polymers, possessing compositionally dependent trends in thermal...

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Bibliographic Details
Published in:Biomacromolecules 2016-10, Vol.17 (10), p.3396-3403
Main Authors: Childers, Erin P, Peterson, Gregory I, Ellenberger, Alex B, Domino, Karen, Seifert, Gabrielle V, Becker, Matthew L
Format: Article
Language:English
Subjects:
Adsorption - drug effects
Animals
Aorta - cytology
Aorta - drug effects
Aorta - growth & development
Cattle
Fibrinogen - chemistry
Fibrinogen - pharmacology
Platelet-Rich Plasma - chemistry
Polyesters - chemistry
Polyesters - pharmacology
Quartz Crystal Microbalance Techniques
Rats
Serum Albumin, Bovine - chemistry
Serum Albumin, Bovine - pharmacology
Surface Properties - drug effects
Urea - analogs & derivatives
Urea - chemistry
Urea - pharmacology
Valine - chemistry
Valine - pharmacology
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Summary:The competitive absorption of blood plasma components including fibrinogen (FG), bovine serum albumin (BSA), and platelet-rich plasma (PRP) on l -valine-based poly­(ester urea) (PEU) surfaces were investigated. Using four different PEU polymers, possessing compositionally dependent trends in thermal, mechanical, and critical surface tension measurements, water uptake studies were carried out to determine in vitro behavior of the materials. Quartz crystal microbalance (QCM) measurements were used to quantify the adsorption characteristics of PRP onto PEU thin films by coating the surfaces initially with FG or BSA. Pretreatment of the PEU surfaces with FG inhibited the adsorption of PRP and BSA decreased the absorption 4-fold. In vitro studies demonstrated that cells cultured on l -valine-based PEU thin films allowed attachment and spreading of rat aortic cells. These measurements will be critical toward efforts to use this new class of materials in blood-contacting biomaterials applications.
ISSN:1525-7797
1526-4602
DOI:10.1021/acs.biomac.6b01195