Effects of choline treatment in concentrations of serum matrix metalloproteinases (MMPs), MMP tissue inhibitors (TIMPs) and immunoglobulins in an experimental model of canine sepsis

•MMP and TIMP panels increased significantly at 1–48h after LPS administration.•Serum MMPs and TIMPs are involved in the pathophysiology of endotoxemia, and are correlated with the acute phase reaction (Serum CRP) and multi-organ failure (muscle and hepatorenal).•Intravenous choline treatment reduce...

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Published in:Veterinary immunology and immunopathology 2016-11, Vol.180, p.9-14
Main Authors: Kocaturk, Meric, Eralp-Inan, Oya, Tvarijonaviciute, A., Cansev, Mehmet, Ozyigit, M. Ozgur, Ceron, J.J., Yilmaz, Zeki, Kahraman, M. Mufit
Format: Article
Language:English
Subjects:
Animals
C-Reactive Protein - analysis
Choline
Choline - therapeutic use
Disease Models, Animal
Dog
Dogs
Endotoxemia
Endotoxemia - drug therapy
Endotoxemia - immunology
Endotoxemia - metabolism
Immunoglobulin
Immunoglobulin G - blood
Immunoglobulin M - blood
Matrix metalloproteinase
Matrix Metalloproteinases - blood
Sepsis
Tissue Inhibitor of Metalloproteinases - blood
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Summary:•MMP and TIMP panels increased significantly at 1–48h after LPS administration.•Serum MMPs and TIMPs are involved in the pathophysiology of endotoxemia, and are correlated with the acute phase reaction (Serum CRP) and multi-organ failure (muscle and hepatorenal).•Intravenous choline treatment reduced changes in serum MMPs and TIMPs in dogs with endotoxemia. The aim of the present study was to investigate effects of intravenous (i.v.) choline treatment on serum matrix metalloproteinases (MMP), MMP tissue inhibitors (TIMP) and immunoglobulins (Igs), and to determine if there were relations between serum MMPs/TIMPs and C-reactive protein (CRP) (as a marker of the acute phase response), immunoglobulin G and M (IgG and IgM) (as a maker of the Ig responses) and markers of organ damage such as muscular damage (creatine phosphokinase, [CPK]), liver damage (alanine aminotransferase [ALT]) and renal dysfunction (blood urea nitrogen [BUN] and creatinine, [Cr]) in dogs with endotoxemia. Healthy dogs (n=24) were randomized to Saline, Choline (C), Lipopolysaccharide (LPS), and LPS+C groups and received 0.9% NaCl (5mL/i.v.), choline chloride (20mg/kg/i.v.), LPS (0.02mg/kg/i.v.) and LPS (0.02mg/kg/i.v.) plus choline chloride (20mg/kg/i.v.), respectively. Serum MMPs and TIMPs concentrations were analyzed by commercial ELISA kits. MMP and TIMP increased at 1–48h (P
ISSN:0165-2427
1873-2534
DOI:10.1016/j.vetimm.2016.08.011