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Deletion of pilA, a Minor Pilin-Like Gene, from Xanthomonas citri subsp. citri Influences Bacterial Physiology and Pathogenesis
Type IV pili (Tfp) are widely distributed adhesins of bacterial surfaces. In plant pathogenic bacteria, Tfp are involved in host colonization and pathogenesis. Xanthomonas citri subsp. citri (Xcc) is the phytopathogen responsible for citrus canker disease. In this work, three Tfp structural genes, f...
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| Published in: | Current microbiology 2016-12, Vol.73 (6), p.904-914 |
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| container_end_page | 914 |
| container_issue | 6 |
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| container_title | Current microbiology |
| container_volume | 73 |
| creator | Petrocelli, Silvana Arana, Maite R. Cabrini, Marcela N. Casabuono, Adriana C. Moyano, Laura Beltramino, Matías Moreira, Leandro M. Couto, Alicia S. Orellano, Elena G. |
| description | Type IV pili (Tfp) are widely distributed adhesins of bacterial surfaces. In plant pathogenic bacteria, Tfp are involved in host colonization and pathogenesis.
Xanthomonas citri
subsp.
citri
(Xcc) is the phytopathogen responsible for citrus canker disease. In this work, three Tfp structural genes,
fimA
,
fimA1
, and
pilA
from Xcc were studied. A
pilA
mutant strain from Xcc (
XccΔpilA
) was constructed and differences in physiological features, such as motilities, adhesion, and biofilm formation, were observed. A structural study of the purified Tfp fractions from Xcc wild-type and
Xcc∆pilA
showed that pilins are glycosylated in both strains and that FimA and FimA1 are the main structural components of the pili. Furthermore, smaller lesion symptoms and reduced bacterial growth were produced by
Xcc∆pilA
in orange plants compared to the wild-type strain. These results indicate that the minor pilin-like gene,
pilA
, is involved in Tfp performance during the infection process. |
| doi_str_mv | 10.1007/s00284-016-1138-1 |
| format | article |
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Xanthomonas citri
subsp.
citri
(Xcc) is the phytopathogen responsible for citrus canker disease. In this work, three Tfp structural genes,
fimA
,
fimA1
, and
pilA
from Xcc were studied. A
pilA
mutant strain from Xcc (
XccΔpilA
) was constructed and differences in physiological features, such as motilities, adhesion, and biofilm formation, were observed. A structural study of the purified Tfp fractions from Xcc wild-type and
Xcc∆pilA
showed that pilins are glycosylated in both strains and that FimA and FimA1 are the main structural components of the pili. Furthermore, smaller lesion symptoms and reduced bacterial growth were produced by
Xcc∆pilA
in orange plants compared to the wild-type strain. These results indicate that the minor pilin-like gene,
pilA
, is involved in Tfp performance during the infection process.</description><identifier>ISSN: 0343-8651</identifier><identifier>EISSN: 1432-0991</identifier><identifier>DOI: 10.1007/s00284-016-1138-1</identifier><identifier>PMID: 27664015</identifier><language>eng</language><publisher>New York: Springer US</publisher><subject>Adhesins ; Bacteria ; Bacterial physiology ; Bacterial Proteins - genetics ; Bacterial Proteins - metabolism ; Biofilms ; Biomedical and Life Sciences ; Biotechnology ; Canker ; Citrus - microbiology ; Colonization ; Fimbriae Proteins - genetics ; Fimbriae Proteins - metabolism ; Gene Deletion ; Genes ; Host plants ; Life Sciences ; Microbiology ; Pathogenesis ; Physiology ; PilA protein ; Pili ; Pilin ; Plant bacterial diseases ; Plant Diseases - microbiology ; Plants (botany) ; Virulence ; Xanthomonas - genetics ; Xanthomonas - metabolism ; Xanthomonas - pathogenicity ; Xanthomonas citri</subject><ispartof>Current microbiology, 2016-12, Vol.73 (6), p.904-914</ispartof><rights>Springer Science+Business Media New York 2016</rights><rights>Current Microbiology is a copyright of Springer, 2016.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c372t-707693b7940e98a5eea18762736079058213ba7c3040c80580c9899023859f563</citedby><cites>FETCH-LOGICAL-c372t-707693b7940e98a5eea18762736079058213ba7c3040c80580c9899023859f563</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/27664015$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Petrocelli, Silvana</creatorcontrib><creatorcontrib>Arana, Maite R.</creatorcontrib><creatorcontrib>Cabrini, Marcela N.</creatorcontrib><creatorcontrib>Casabuono, Adriana C.</creatorcontrib><creatorcontrib>Moyano, Laura</creatorcontrib><creatorcontrib>Beltramino, Matías</creatorcontrib><creatorcontrib>Moreira, Leandro M.</creatorcontrib><creatorcontrib>Couto, Alicia S.</creatorcontrib><creatorcontrib>Orellano, Elena G.</creatorcontrib><title>Deletion of pilA, a Minor Pilin-Like Gene, from Xanthomonas citri subsp. citri Influences Bacterial Physiology and Pathogenesis</title><title>Current microbiology</title><addtitle>Curr Microbiol</addtitle><addtitle>Curr Microbiol</addtitle><description>Type IV pili (Tfp) are widely distributed adhesins of bacterial surfaces. In plant pathogenic bacteria, Tfp are involved in host colonization and pathogenesis.
Xanthomonas citri
subsp.
citri
(Xcc) is the phytopathogen responsible for citrus canker disease. In this work, three Tfp structural genes,
fimA
,
fimA1
, and
pilA
from Xcc were studied. A
pilA
mutant strain from Xcc (
XccΔpilA
) was constructed and differences in physiological features, such as motilities, adhesion, and biofilm formation, were observed. A structural study of the purified Tfp fractions from Xcc wild-type and
Xcc∆pilA
showed that pilins are glycosylated in both strains and that FimA and FimA1 are the main structural components of the pili. Furthermore, smaller lesion symptoms and reduced bacterial growth were produced by
Xcc∆pilA
in orange plants compared to the wild-type strain. These results indicate that the minor pilin-like gene,
pilA
, is involved in Tfp performance during the infection process.</description><subject>Adhesins</subject><subject>Bacteria</subject><subject>Bacterial physiology</subject><subject>Bacterial Proteins - genetics</subject><subject>Bacterial Proteins - metabolism</subject><subject>Biofilms</subject><subject>Biomedical and Life Sciences</subject><subject>Biotechnology</subject><subject>Canker</subject><subject>Citrus - microbiology</subject><subject>Colonization</subject><subject>Fimbriae Proteins - genetics</subject><subject>Fimbriae Proteins - metabolism</subject><subject>Gene Deletion</subject><subject>Genes</subject><subject>Host plants</subject><subject>Life Sciences</subject><subject>Microbiology</subject><subject>Pathogenesis</subject><subject>Physiology</subject><subject>PilA protein</subject><subject>Pili</subject><subject>Pilin</subject><subject>Plant bacterial diseases</subject><subject>Plant Diseases - microbiology</subject><subject>Plants (botany)</subject><subject>Virulence</subject><subject>Xanthomonas - genetics</subject><subject>Xanthomonas - metabolism</subject><subject>Xanthomonas - pathogenicity</subject><subject>Xanthomonas citri</subject><issn>0343-8651</issn><issn>1432-0991</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2016</creationdate><recordtype>article</recordtype><recordid>eNp1kUFvFCEUgInR2G31B3gxJF48dOp7wAxwrLXWJmvcgybeCEuZLXUGtjBz2JN_XTa7GmPiibzw8fGSj5BXCBcIIN8VAKZEA9g1iFw1-IQsUHDWgNb4lCyAC96orsUTclrKAwAyDficnDDZdQKwXZCfH_zgp5AiTT3dhuHynFr6OcSU6SoMITbL8MPTGx_9Oe1zGul3G6f7NKZoC3VhyoGWeV22F8fhNvbD7KPzhb63bvI52IGu7nclpCFtdtTGO7qy1bCpyhLKC_Kst0PxL4_nGfn28frr1adm-eXm9upy2Tgu2dRIkJ3ma6kFeK1s671FJTsmeQdSQ6sY8rWVjoMAp-oMTiutgXHV6r7t-Bl5e_Buc3qcfZnMGIrzw2CjT3MxqHgrsFUdVvTNP-hDmnOs2xnUTAoplJCVwgPlciol-95scxht3hkEs69jDnVMrWP2dcze_Ppontejv_vz4neOCrADUOpV3Pj819f_tf4CM0uXvw</recordid><startdate>20161201</startdate><enddate>20161201</enddate><creator>Petrocelli, Silvana</creator><creator>Arana, Maite R.</creator><creator>Cabrini, Marcela N.</creator><creator>Casabuono, Adriana C.</creator><creator>Moyano, Laura</creator><creator>Beltramino, Matías</creator><creator>Moreira, Leandro M.</creator><creator>Couto, Alicia S.</creator><creator>Orellano, Elena G.</creator><general>Springer US</general><general>Springer Nature B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QL</scope><scope>7T7</scope><scope>7TK</scope><scope>7TM</scope><scope>7U9</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>8AO</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>8G5</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>GUQSH</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M2O</scope><scope>M7N</scope><scope>M7P</scope><scope>MBDVC</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>Q9U</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>20161201</creationdate><title>Deletion of pilA, a Minor Pilin-Like Gene, from Xanthomonas citri subsp. citri Influences Bacterial Physiology and Pathogenesis</title><author>Petrocelli, Silvana ; Arana, Maite R. ; Cabrini, Marcela N. ; Casabuono, Adriana C. ; Moyano, Laura ; Beltramino, Matías ; Moreira, Leandro M. ; Couto, Alicia S. ; Orellano, Elena G.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c372t-707693b7940e98a5eea18762736079058213ba7c3040c80580c9899023859f563</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2016</creationdate><topic>Adhesins</topic><topic>Bacteria</topic><topic>Bacterial physiology</topic><topic>Bacterial Proteins - 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Academic</collection><jtitle>Current microbiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Petrocelli, Silvana</au><au>Arana, Maite R.</au><au>Cabrini, Marcela N.</au><au>Casabuono, Adriana C.</au><au>Moyano, Laura</au><au>Beltramino, Matías</au><au>Moreira, Leandro M.</au><au>Couto, Alicia S.</au><au>Orellano, Elena G.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Deletion of pilA, a Minor Pilin-Like Gene, from Xanthomonas citri subsp. citri Influences Bacterial Physiology and Pathogenesis</atitle><jtitle>Current microbiology</jtitle><stitle>Curr Microbiol</stitle><addtitle>Curr Microbiol</addtitle><date>2016-12-01</date><risdate>2016</risdate><volume>73</volume><issue>6</issue><spage>904</spage><epage>914</epage><pages>904-914</pages><issn>0343-8651</issn><eissn>1432-0991</eissn><abstract>Type IV pili (Tfp) are widely distributed adhesins of bacterial surfaces. In plant pathogenic bacteria, Tfp are involved in host colonization and pathogenesis.
Xanthomonas citri
subsp.
citri
(Xcc) is the phytopathogen responsible for citrus canker disease. In this work, three Tfp structural genes,
fimA
,
fimA1
, and
pilA
from Xcc were studied. A
pilA
mutant strain from Xcc (
XccΔpilA
) was constructed and differences in physiological features, such as motilities, adhesion, and biofilm formation, were observed. A structural study of the purified Tfp fractions from Xcc wild-type and
Xcc∆pilA
showed that pilins are glycosylated in both strains and that FimA and FimA1 are the main structural components of the pili. Furthermore, smaller lesion symptoms and reduced bacterial growth were produced by
Xcc∆pilA
in orange plants compared to the wild-type strain. These results indicate that the minor pilin-like gene,
pilA
, is involved in Tfp performance during the infection process.</abstract><cop>New York</cop><pub>Springer US</pub><pmid>27664015</pmid><doi>10.1007/s00284-016-1138-1</doi><tpages>11</tpages></addata></record> |
| fulltext | fulltext |
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| ispartof | Current microbiology, 2016-12, Vol.73 (6), p.904-914 |
| issn | 0343-8651 1432-0991 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_1835415861 |
| source | Springer Nature:Jisc Collections:Springer Nature Read and Publish 2023-2025: Springer Reading List |
| subjects | Adhesins Bacteria Bacterial physiology Bacterial Proteins - genetics Bacterial Proteins - metabolism Biofilms Biomedical and Life Sciences Biotechnology Canker Citrus - microbiology Colonization Fimbriae Proteins - genetics Fimbriae Proteins - metabolism Gene Deletion Genes Host plants Life Sciences Microbiology Pathogenesis Physiology PilA protein Pili Pilin Plant bacterial diseases Plant Diseases - microbiology Plants (botany) Virulence Xanthomonas - genetics Xanthomonas - metabolism Xanthomonas - pathogenicity Xanthomonas citri |
| title | Deletion of pilA, a Minor Pilin-Like Gene, from Xanthomonas citri subsp. citri Influences Bacterial Physiology and Pathogenesis |
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