Diagnosis and prevalence of Theileria equi horses in western Mexico by nested PCR

Abstract Theileria equi infection prevalence was calculated from 1 000 blood samples obtained from apparently healthy horses in western Mexico. Samples were sent to the Animal Biotechnology Laboratory of the University of Guadalajara (Mexico) for T. equi diagnosis. Nested polymerase chain reaction (...

Full description

Saved in:
Bibliographic Details
Published in:Parasitology international 2017-02, Vol.66 (1), p.821-824
Main Authors: Ayala-Valdovinos, Miguel Angel, Lemus-Flores, Clemente, Galindo-García, Jorge, Bañuelos-Pineda, Jacinto, Rodríguez-Carpena, Javier Germán, Sánchez-Chiprés, David, Duifhuis-Rivera, Theodor
Format: Article
Language:English
Subjects:
Animals
Babesiosis - epidemiology
Babesiosis - parasitology
Female
Gastroenterology and Hepatology
Horse
Horse Diseases - diagnosis
Horse Diseases - epidemiology
Horse Diseases - parasitology
Horses - parasitology
Infectious Disease
Male
Mexico
Mexico - epidemiology
Nested polymerase chain reaction
Polymerase Chain Reaction - veterinary
Prevalence
Theileria - genetics
Theileria - isolation & purification
Theileria equi
Theileriasis - diagnosis
Theileriasis - epidemiology
Theileriasis - parasitology
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Abstract Theileria equi infection prevalence was calculated from 1 000 blood samples obtained from apparently healthy horses in western Mexico. Samples were sent to the Animal Biotechnology Laboratory of the University of Guadalajara (Mexico) for T. equi diagnosis. Nested polymerase chain reaction (nPCR) was used as a diagnostic method to detect pathogen DNA. Using primers for the merozoite antigen-1 (EMA-1) gene, 19.70 ± 2.47% of the horses (95% CI, 17.23–22.17%) tested positive for T. equi. There was no significant association between gender and T. equi infection. However, prevalence was higher among stabled horses (25.81%) than that among grazing horses (15.02%). The positivity rate was also higher among Quarter Horse (24.70%), Lusitano (35.90%), and Costa Rican Saddle Horse (47.37%) breeds than that among the other seven breeds investigated in this study. The percentage of T. equi infection was higher among adult horses (≥ 4 years old, 25.05%) than that among colts and fillies (2–4 years old, 15.48%), yearlings (1–2 years old, 10.49%), and foals (< 1 year old, 10.34%). This is the first study of T. equi infection prevalence among horses in Mexico by nPCR . The results indicate that the equine piroplasmosis (EP) caused by T. equi is enzootic in western Mexico.
ISSN:1383-5769
1873-0329
DOI:10.1016/j.parint.2016.09.011