Development of a real-time PCR for Bartonella spp. detection, a current emerging microorganism

A real-time PCR assay using SYBR Green was optimized to detect those Bartonella that are most frequently described as pathogens. The assay was genus-specific. Sequencing allowed to distinguish species. Assay sensitivity was determined using 10-fold serial dilutions of genomic DNA. Dynamic range was...

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Bibliographic Details
Published in:Molecular and cellular probes 2017-04, Vol.32, p.55-59
Main Authors: Parra, Elena, Segura, Ferran, Tijero, Jessica, Pons, Imma, Nogueras, Maria-Mercedes
Format: Article
Language:English
Subjects:
Bartonella
Bartonella - genetics
Bartonella - isolation & purification
Base Sequence
Optimization
Real-time PCR
Real-Time Polymerase Chain Reaction - methods
Sensitivity and Specificity
Sequence Alignment
Zoonoses
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Summary:A real-time PCR assay using SYBR Green was optimized to detect those Bartonella that are most frequently described as pathogens. The assay was genus-specific. Sequencing allowed to distinguish species. Assay sensitivity was determined using 10-fold serial dilutions of genomic DNA. Dynamic range was 100 ng–100 fg and sensitivity was 50 copies/reaction. •A real-time PCR assay to detect Bartonella spp. species has been developed.•This assay has been tested with the most frequent species of Bartonella spp.•Its flexibility may allow to detect more species, even those not described yet.
ISSN:0890-8508
1096-1194
DOI:10.1016/j.mcp.2016.10.006