Relationship Between Serum microRNA155 and Telomerase Expression in Hepatocellular Carcinoma

Background and Aims Activation of telomerase reverse transcriptase (hTERT) has a role in liver carcinogenesis where telomerase is normally suppressed in most human somatic tissues after birth. In the current study we aimed to detect the significance of hTERT mRNA in early detection of hepatocellular...

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Bibliographic Details
Published in:Archives of medical research 2016-07, Vol.47 (5), p.349-355
Main Authors: Ezzat, Wafaa M, Amr, Khalda Said, Raouf, Haiam Abdel, Elhosary, Yasser A, Hegazy, Abdelfattah E, Fahim, Hoda H, Kamel, Refaat R
Format: Article
Language:English
Subjects:
Adult
Carcinoma, Hepatocellular - metabolism
Case-Control Studies
Female
hTERT-HCC
Humans
Internal Medicine
Liver Cirrhosis - blood
Liver Cirrhosis - metabolism
Liver Neoplasms - blood
Liver Neoplasms - metabolism
Male
MicroRNAs - blood
Middle Aged
miR 155
RNA, Messenger
Telomerase
Telomerase - genetics
Telomerase - metabolism
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Summary:Background and Aims Activation of telomerase reverse transcriptase (hTERT) has a role in liver carcinogenesis where telomerase is normally suppressed in most human somatic tissues after birth. In the current study we aimed to detect the significance of hTERT mRNA in early detection of hepatocellular carcinoma (HCC) and to determine the relationship between serum microRNA155 and telomerase expression. Methods Serum and liver tissue samples were collected from 40 patients (17 samples from patients with liver cirrhosis and 23 samples from patients with HCC) and 12 blood samples from healthy subjects were collected. Serum miRNA155 levels and blood and tissue hTERT mRNA were detected by real-time quantitative reverse-transcriptase PCR (RT-qPCR) among liver cirrhosis and HCC patients. Moreover, miR-155, blood and tissue hTERT levels were analyzed in relation to clinical and pathological features. Results Calculated expression of miRNA155 revealed that relative quantity (RQ) miR 155 was overexpressed in sera of HCC patients when compared to patients with liver cirrhosis and controls ( p  
ISSN:0188-4409
1873-5487
DOI:10.1016/j.arcmed.2016.08.003