Extracellular vesicles as a source for non-invasive biomarkers in bladder cancer progression

Bladder cancer is the second most frequent malignancy of the urinary tract after prostate cancer. Current diagnostic techniques, such as cystoscopy and biopsies are highly invasive and accompanied of undesirable side effects. Moreover, there are no suitable biomarkers for relapse or progression prog...

Full description

Saved in:
Bibliographic Details
Published in:European journal of pharmaceutical sciences 2017-02, Vol.98, p.70-79
Main Authors: Andreu, Zoraida, Otta Oshiro, Renan, Redruello, Alberto, López-Martín, Soraya, Gutiérrez-Vázquez, Cristina, Morato, Esperanza, Marina, Ana Isabel, Olivier Gómez, Carlos, Yáñez-Mó, María
Format: Article
Language:English
Subjects:
Adult
Aged
Aged, 80 and over
Apo B
Apolipoprotein B-100 - urine
Biomarkers, Tumor - urine
Bladder cancer
Cell Line, Tumor
Disease Progression
Extracellular vesicles
Extracellular Vesicles - metabolism
Female
Humans
Male
MicroRNAs
MicroRNAs - urine
Middle Aged
Proteomics
Urinary Bladder Neoplasms - urine
Urine
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Bladder cancer is the second most frequent malignancy of the urinary tract after prostate cancer. Current diagnostic techniques, such as cystoscopy and biopsies are highly invasive and accompanied of undesirable side effects. Moreover, there are no suitable biomarkers for relapse or progression prognosis. We analysed whether the specific composition of microRNAs (miRNAs) and proteins of extracellular vesicles (EVs) that urothelial tumour cells of bladder mucosa release into the urine, could reflect their pathologic condition. For this purpose, urinary EVs were isolated and their protein and miRNA composition evaluated in healthy donors and low or high-grade bladder cancer patients. Using a microarray platform containing probes for 851 human miRNAs we found 26 deregulated miRNAs in high-grade bladder cancer urine EVs, from which 23 were downregulated and 3 upregulated. Real-time PCR analysis pointed to miR-375 as a biomarker for high-grade bladder cancer while miR-146a could identify low-grade patients. Finally, several protein markers were also deregulated in EVs from tumour patients. Our data suggest that the presence of ApoB in the 100,000 pellet is a clear marker for malignancy. [Display omitted]
ISSN:0928-0987
1879-0720
DOI:10.1016/j.ejps.2016.10.008