Application of rapid CD3 immunophenotype analysis and argyrophilic nucleolar organizer region (AgNOR) frequency to fine needle aspirate specimens from dogs with Lymphoma

Determinations of CD3 immunoreactivity (CD3‐IR) and argyrophilic nucleolar organizer region (AgNOR) frequency from fine needle aspirate (FNA) samples were compared with those from formalinfixed, paraffin‐embedded surgical biopsy samples in 51 dogs with lymphoma. Both CD3‐IR (using a rapid EPOSR poly...

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Bibliographic Details
Published in:Veterinary clinical pathology 1997-01, Vol.26 (2), p.66-70
Main Authors: Vail, D.M. (University of Wisconsin-Madison, Madison, WI.), Kravis, L.D, Kisseberth, W.C, Ogilvie, G.K, Volk, L.M
Format: Article
Language:English
Subjects:
ANALYTICAL METHODS
ANTIGENE
ANTIGENOS
ANTIGENS
canine
CD3
CHEMISTRY
CHIEN
CHIMIE
DIAGNOSIS
DIAGNOSTIC
DIAGNOSTICO
DOGS
FINE NEEDLE ASPIRATION
FORECASTING
IMMUNOHISTOCHEMISTRY
IMMUNOLOGIE
IMMUNOLOGY
immunophenotype
INMUNOLOGIA
LINFOMA
LYMPHOMA
LYMPHOME
nucleolar organizer region
NUCLEOLE
NUCLEOLOS
NUCLEOLUS
NUCLEOLUS ORGANIZER
PATHOGENESE
PATHOGENESIS
PATOGENESIS
PERRO
QUIMICA
RAPID METHODS
REMISSION
SURFACE ANTIGENS
TECHNIQUE ANALYTIQUE
TECHNIQUE DE PREVISION
TECNICAS ANALITICAS
TECNICAS DE PREDICCION
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Summary:Determinations of CD3 immunoreactivity (CD3‐IR) and argyrophilic nucleolar organizer region (AgNOR) frequency from fine needle aspirate (FNA) samples were compared with those from formalinfixed, paraffin‐embedded surgical biopsy samples in 51 dogs with lymphoma. Both CD3‐IR (using a rapid EPOSR polymer system) and AgNOR techniques were readily applied to FNA samples. CD3‐IR from FNA samples matched those of histologic samples with 100% concordance. A linear relationship (r = 0.981; P < 0.001>) was found between AgNOR frequency obtained from FNA samples and those obtained from surgical biopsy samples. Application of the techniques presented here should allow clinically relevant information to be procured rapidly and inexpensively. As CD3‐IR and AgNOR frequency have been shown to be predictive of response to combination chemotherapy in dogs with lymphoma, such information could be used to better educate clients as to the likelihood of achieving meaningful responses, as well as allowing prospective tailoring of individual treatments in future trials prior to initiating therapy.
ISSN:0275-6382
1939-165X
DOI:10.1111/j.1939-165X.1997.tb00711.x