Implications of leaf ontogeny on drought-induced gradients of CAM expression and ABA levels in rosettes of the epiphytic tank bromeliad Guzmania monostachia

• Published in: Plant physiology and biochemistry 2016-11, Vol.108, p.400-411
• Main Authors: Rodrigues, Maria Aurineide, Hamachi, Leonardo, Mioto, Paulo Tamaso, Purgatto, Eduardo, Mercier, Helenice
• Format: Article
• Language: English
• Subjects: ABA; Abscisic Acid - metabolism; Bromeliaceae - metabolism; Bromeliaceae - physiology; CAM; Drought; Droughts; Epiphytes; Guzmania monostachia; Leaf ontogeny; Photosynthesis; Plant Leaves - anatomy & histology; Plant Leaves - metabolism; Rosette plants; Tank bromeliad; Up-Regulation; Water - metabolism
• ISSN: 0981-9428; 1873-2690
• DOI: 10.1016/j.plaphy.2016.08.010

Guzmania monostachia is an epiphytic heteroblastic bromeliad that exhibits rosette leaves forming water-holding tanks at maturity. Different portions along its leaf blades can display variable degrees of crassulacean acid metabolism (CAM) up-regulation under drought. Since abscisic acid (ABA) can act as an important long-distance signal, we conducted a joint investigation of ontogenetic and drought impacts on CAM intensity and ABA levels in different leaf groups within the G. monostachia rosette. For this, three groups of leaves were analysed according to their position within the mature-tank rosette (i.e., younger, intermediate, and older leaves) to characterize the general growth patterns and magnitude of drought-modulated CAM expression. CAM activity was evaluated by analysing key molecules in the biochemical machinery of this photosynthetic pathway, while endogenous ABA content was comparatively measured in different portions of each leaf group after seven days under well-watered (control) or drought treatment. The results revealed that G. monostachia shows more uniform morphological characteristics along the leaves when in the atmospheric stage. The drought treatment of mature-tank rosettes generally induced in older leaves a more severe water loss, followed by the lowest CAM activity and a higher increase in ABA levels, while younger leaves showed an opposite response. Therefore, leaf groups at distinct ontogenetic stages within the tank rosette of G. monostachia responded to drought with variable degrees of water loss and CAM expression. ABA seems to participate in this tissue-compartmented response as a long-distance signalling molecule, transmitting the drought-induced signals originated in older leaves towards the younger ones. •G. monostachia rosette has a well-defined spatial gradient of leaf ontogenesis.•Leaves of mature-tank rosettes display a higher degree of morphological variation.•Drought-induced responses in tank rosettes depend on leaf arrangement/ontogeny.•Younger leaves lost less water and performed stronger CAM under water shortage.•ABA may act as a systemic signal in the rosette, transported towards younger leaves.