Characterisation, in vitro release study, and antibacterial activity of montmorillonite-gentamicin complex material

• Published in: Materials Science & Engineering C 2017-01, Vol.70 (Pt 1), p.471-478
• Main Authors: Rapacz-Kmita, A., Bućko, M.M., Stodolak-Zych, E., Mikołajczyk, M., Dudek, P., Trybus, M.
• Format: Article
• Language: English
• Subjects: Anti-Bacterial Agents - pharmacology; Antibacterial activity; Bentonite - chemistry; Controlled drug release; Drug Liberation; Escherichia coli - drug effects; Gentamicin; Gentamicins - pharmacology; Microbial Sensitivity Tests; Montmorillonite; Spectroscopy, Fourier Transform Infrared; X-Ray Diffraction; X-ray methods
• ISSN: 0928-4931; 1873-0191
• DOI: 10.1016/j.msec.2016.09.031

The present paper concerns the potential use of montmorillonite as a drug carrier and focusses on the intercalation of the studied clay with gentamicin (an aminoglycoside antibiotic) at various temperatures (20, 50 and 80°C). The experiments were performed to identify the temperature required for the optimum intercalation of gentamicin into the interlayer of montmorillonite. The structural and microstructural properties of gentamicin and the potential for introducing it between smectite clay layers were investigated by means of X-ray diffraction (XRD), Fourier transform infrared (FTIR) spectroscopic techniques, and SEM with EDS analysis. Additionally, the in vitro drug release behaviour of the montmorillonite-gentamicin complex and its antibacterial activity against Escherichia coli (E. coli) bacteria was investigated. Based on these studies, the impact of temperature on the intercalation of the drug between layers of smectite was evaluated. It was found that an intercalation temperature of 50°C resulted in the highest shift in the position of principle peak d(001) as measured by XRD, suggesting, that the greatest amount of gentamicin had been introduced into the interlayer space of montmorillonite at this temperature. Subsequently, the montmorillonite-gentamicin complex material obtained at 50°C revealed the greatest capacity for killing E. coli bacteria during an in vitro test. •A novel montmorillonite-gentamicin hybrid materials was prepared as potential drug carrier.•Optimal conditions for the intercalation of gentamicin into the interlayer space of montmorillonite were tested.•The MMT-G complex material obtained at 50°C revealed the greatest capacity for killing E. coli during the inhibitory zone test.•Modulating drug delivery was monitored and confirmed in in vitro drug release study.