A label-free, PCR-free and signal-on electrochemical DNA biosensor for Leishmania major based on gold nanoleaves

• Published in: Talanta (Oxford) 2016-12, Vol.161, p.48-53
• Main Authors: Moradi, M., Sattarahmady, N., Rahi, A., Hatam, G.R., Sorkhabadi, S.M. Rezayat, Heli, H.
• Format: Article
• Language: English
• Subjects: DNA biosensor; Genosensor; Gold nanoleaves; Leishmaniasis
• ISSN: 0039-9140; 1873-3573
• DOI: 10.1016/j.talanta.2016.08.030

Detection of leishmaniasis is important in clinical diagnoses. In the present study, identification of Leishmania parasites was performed by a label-free, PCR-free and signal-on ultrasensitive electrochemical DNA biosensor. Gold nanoleaves were firstly electrodeposited by an electrodeposition method using spermidine as a shape directing agent. The biosensor was fabricated by immobilization of a Leishmania major specific DNA probe onto gold nanoleaves, and methylene blue was employed as a marker. Hybridization of the complementary single stranded DNA sequence with the biosensor under the selected conditions was then investigated. The biosensor could detect a synthetic DNA target in a range of 1.0×10−10 to 1.0×10−19molL−1 with a limit of detection of 1.8×10−20molL−1, and genomic DNA in a range of 0.5–20ngμL−1 with a limit of detection of 0.07ngμL−1. The biosensor could distinguish Leishmania major from a non-complementary-sequence oligonucleotide and the tropica species with a high selectivity. The biosensor was applicable to detect Leishmania major in patient samples. [Display omitted] ●Gold nanoleaves were electrodeposited by electrodeposition.●A Leishmania major specific DNA probe onto gold nanoleaves to fabricate a signal-on ultrasensitive electrochemical DNA biosensor.●The biosensor was applicable to detect Leishmania major in patient samples.