Optical non-contact pH measurement in cell culture with sterilizable, modular parts

• Published in: Talanta (Oxford) 2016-12, Vol.161, p.755-761
• Main Authors: Kattipparambil Rajan, Dhanesh, Patrikoski, Mimmi, Verho, Jarmo, Sivula, Jyrki, Ihalainen, Heimo, Miettinen, Susanna, Lekkala, Jukka
• Format: Article
• Language: English
• Subjects: Adipose Tissue - cytology; Cell culture non-contact pH measurement; Cell culture optical pH measurement; Cell culture pH measurement; Cell Culture Techniques - methods; Female; Fibroblasts; Humans; Hydrogen-Ion Concentration; Indicators and Reagents; Long term pH measurement; Middle Aged; Phenol red medium pH measurement; Phenolsulfonphthalein; Printing, Three-Dimensional; Stem Cells; Sterilization; Temperature
• ISSN: 0039-9140; 1873-3573
• DOI: 10.1016/j.talanta.2016.09.021

A non-contact real time pH measurement using fully modular optical parts is described for phenol-red medium cell cultures. The modular parts can be sterilized, and once the measurement is started at the beginning of culture, no recalibration or maintenance is needed till the end of the culture. Measurements can be carried out without any special manual attention. The modular assembly of LED and sensor cassettes is unique, robust, reusable and reproducible. pH is measured in an intact closed flow system, without wasting any culture medium. A special pump encapsulation enables the system to be effortlessly functional in extremely humid incubator environments. This avoids lengthy sample tubings in and out of the incubator, associated large temperature changes and CO2 buffering issues. A new correction model to compensate errors caused e.g. by biolayers in spectrometric pH measurement is put-forward, which improves the accuracy of pH estimation significantly. The method provides resolution down to 0.1 pH unit in physiological pH range with mean absolute error 0.02. [Display omitted] •Real time non-contact optical pH measurement in phenol red cell culture.•3D printed modular assembly is unique, robust, reusable and reproducible.•Neither sample extraction off the incubator nor special sample treatment is needed.•New absorption correction model improves pH accuracy in long term culture.•Modular parts can be sterilized. Effect of temperature is continuously compensated.