Carnitine supplementation decreases capacitation-like changes of frozen-thawed buffalo spermatozoa

The aim of this study was to evaluate the effect of carnitine supplementation of semen extender on fertility parameters of frozen-thawed buffalo sperm. Buffalo semen was cryopreserved in BioXcell containing 0 (control group), 2.5 and 7.5-mM carnitine. After thawing, viability, motility, membrane int...

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Bibliographic Details
Published in:Theriogenology 2017-01, Vol.88, p.236-243
Main Authors: Longobardi, Valentina, Salzano, Angela, Campanile, Giuseppe, Marrone, Raffaele, Palumbo, Francesco, Vitiello, Milena, Zullo, Gianluigi, Gasparrini, Bianca
Format: Article
Language:English
Subjects:
Animals
ATP content
Buffalo sperm
Buffaloes
Capacitation-like changes
Carnitine
Carnitine - pharmacology
Cell Survival
Cryopreservation - veterinary
Fertilizing ability
Freezing
Male
Oxidative Stress
Semen Preservation - veterinary
Sperm Capacitation - physiology
Sperm Motility - drug effects
Spermatozoa - drug effects
Spermatozoa - physiology
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Summary:The aim of this study was to evaluate the effect of carnitine supplementation of semen extender on fertility parameters of frozen-thawed buffalo sperm. Buffalo semen was cryopreserved in BioXcell containing 0 (control group), 2.5 and 7.5-mM carnitine. After thawing, viability, motility, membrane integrity and capacitation status (assessed by localization of phosphotyrosine-containing proteins and chlortetracycline, chlortetracycline assay) were evaluated. Furthermore, total antioxidant capacity, reactive oxygen species (ROS) and lipid peroxidation levels, as well as adenosine triphosphate (ATP) content and phospholipids concentration were assessed. Finally, in vitro–fertilizing ability was evaluated after heterologous IVF. An increased post-thawing sperm motility and membrane integrity were recorded in both treated groups compared with the control (44.4 ± 3.5, 53.1 ± 3.9, and 52.5 ± 3.6%; P 
ISSN:0093-691X
1879-3231
DOI:10.1016/j.theriogenology.2016.09.031