Bulbophyllum sterile petroleum ether fraction induces apoptosis in vitro and ameliorates tumor progression in vivo

• Published in: Biomedicine & pharmacotherapy 2016-12, Vol.84, p.1419-1427
• Main Authors: Biswas, Subhankar, Pardeshi, Rashmi, Reddy, Neetinkumar D, Shoja, Muhammed Haneefa, Nayak, Pawan G, Setty, M. Manjunath, Pai, K. Sreedhara R
• Format: Article
• Language: English
• Subjects: A549 Cells; Animals; Antineoplastic Agents, Phytogenic - isolation & purification; Antineoplastic Agents, Phytogenic - pharmacology; Antineoplastic Agents, Phytogenic - therapeutic use; Apoptosis; Apoptosis - drug effects; Apoptosis - physiology; Carcinoma, Ehrlich Tumor - drug therapy; Carcinoma, Ehrlich Tumor - metabolism; Carcinoma, Ehrlich Tumor - pathology; Cell cycle; Comet assay; Disease Progression; Ehrlich ascites carcinoma; Female; HCT116 Cells; Humans; Internal Medicine; Medical Education; Mice; Orchidaceae; Petroleum; Phospho-p53; Plant Extracts - isolation & purification; Plant Extracts - pharmacology; Plant Extracts - therapeutic use; Plant Roots
• ISSN: 0753-3322; 1950-6007
• DOI: 10.1016/j.biopha.2016.10.005

Abstract Orchids of the genus Bulbophyllum have been reported to possess antitumor activity. Present study investigated the possible antitumor activity of the active fraction of bulb and root of Bulbophyllum sterile . Alcoholic extract along with petroleum ether, dichloromethane and ethyl acetate fractions were subjected to SRB assay in HCT-116, MDA-MB-231 and A549 cell lines. The active fractions were further evaluated for apoptosis, expression of apoptotic signaling proteins, comet assay and cell cycle analysis. Furthermore, they were assessed for in vivo antitumor activity in Ehrlich ascites carcinoma model. Petroleum fraction of bulbs (PFB) and roots (PFR) was found to be most active in HCT-116 cell lines with IC50 value of 94.2 ± 6.0 and 75.7 ± 9.8, respectively. Apoptosis was evident from acridine orange/ethidium bromide staining along with the expression of phospho-p53 and phospho-Bad. Both PFB and PFR arrested G2 /M phase of the cell cycle with 32.6% and 49.4% arrest, respectively compared to 17.5% arrest with control. An increase in mean life span and hepatic antioxidant levels was observed with PFB and PFR treatment in EAC inoculated mice. The results suggested that the active fractions of bulbs and roots possess anticancer activity likely by inducing apoptosis through phospho-p53 dependent pathway.