Using Single-Cell Amperometry To Reveal How Cisplatin Treatment Modulates the Release of Catecholamine Transmitters during Exocytosis

The pretreatment of cultured pheochromocytoma (PC12) cells with cis‐diamminedichloroplatinum (cisplatin), an anti‐cancer drug, influences the exocytotic ability of the cells in a dose‐dependent manner. Low concentrations of cisplatin stimulate catecholamine release whereas high concentrations inhibi...

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Bibliographic Details
Published in:Angewandte Chemie 2016-07, Vol.128 (31), p.9187-9190
Main Authors: Li, Xianchan, Dunevall, Johan, Ewing, Andrew G.
Format: Article
Language:English
Subjects:
Amperometrie
Catecholamine
Chemistry
Chemotherapie
Cisplatin
Drugs
Electrical measurements
Exocytose
Lipids
Membranes
Porosity
Stability
Transmitters
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Summary:The pretreatment of cultured pheochromocytoma (PC12) cells with cis‐diamminedichloroplatinum (cisplatin), an anti‐cancer drug, influences the exocytotic ability of the cells in a dose‐dependent manner. Low concentrations of cisplatin stimulate catecholamine release whereas high concentrations inhibit it. Single‐cell amperometry reflects that 2 μm cisplatin treatment increases the frequency of exocytotic events and reduces their duration, whereas 100 μm cisplatin treatment decreases the frequency of exocytotic events and increases their duration. Furthermore, the stability of the initial fusion pore that is formed in the lipid membrane during exocytosis is also regulated differentially by different cisplatin concentrations. This study thus suggests that cisplatin influences exocytosis by multiple mechanisms. Die Vorbehandlung von PC12‐Zellen mit Cisplatin beeinflusst die Exocytose‐Fähigkeit der Zellen in dosisabhängiger Weise. Durch Einzelzell‐Amperometrie wurde gezeigt, dass niedrige Cisplatin‐Konzentrationen die Freisetzung von Catecholamin induzieren, während hohe Konzentrationen diese inhibieren.
ISSN:0044-8249
1521-3757
DOI:10.1002/ange.201602977