Simultaneous determination of 6-mercaptopruine, 6-thioguanine and dasatinib as three important anticancer drugs using nanostructure voltammetric sensor employing Pt/MWCNTs and 1-butyl-3-methylimidazolium hexafluoro phosphate

• Published in: Biosensors & bioelectronics 2016-12, Vol.86, p.879-884
• Main Authors: Karimi-Maleh, Hassan, Shojaei, Abdollah Fallah, Tabatabaeian, Khalil, Karimi, Fatemeh, Shakeri, Shahryar, Moradi, Reza
• Format: Article
• Language: English
• Subjects: 6-Mercaptopurine; 6-Thioguanine; Antineoplastic Agents - analysis; Antineoplastic Agents - chemistry; Antineoplastic Agents - urine; Biosensors; Carbon; Complex Mixtures - analysis; Complex Mixtures - chemistry; Conductometry - instrumentation; Dasatinib; Dasatinib - analysis; Dasatinib - chemistry; Dasatinib - urine; Drugs; Electrodes; Equipment Design; Equipment Failure Analysis; Human body; Humans; Imidazoles - chemistry; Ionic liquid; Mercaptopurine - analysis; Mercaptopurine - chemistry; Mercaptopurine - urine; Metal Nanoparticles - chemistry; Metal Nanoparticles - ultrastructure; Nanostructure; Nanotubes, Carbon - chemistry; Nanotubes, Carbon - ultrastructure; Phosphates; Platinum - chemistry; Pt/MWCNTs; Reproducibility of Results; Sensitivity and Specificity; Sensor; Sensors; Thioguanine - analysis; Thioguanine - chemistry; Thioguanine - urine
• ISSN: 0956-5663; 1873-4235
• DOI: 10.1016/j.bios.2016.07.086

6-Mercaptopurine, 6-thioguanine and dasatinib are three important anticancer drugs with high adverse effects in human body. In this study, a Pt/MWCNTs-1-butyl-3-methylimidazolium hexafluoro phosphate-modified carbon paste electrode was developed for the simultaneously determination of 6-mercaptopurine, 6-thioguanine and dasatinib for the first time. The Pt/MWCNTs synthesized by polyol method and have been characterized by transmission electron microscopy and X-ray diffraction methods. The obtained data revealed that the electro-oxidation of 6-mercaptopurine, 6-thioguanine and dasatinib is facilitated as a novel voltammetric sensor. After optimization of electrochemical parameters employing this sensor at pH 8.0, the oxidation peak currents for 6-mercaptopurine, 6-thioguanine and dasatinib were found to vary linearly with their concentrations in the range of 0.05–550μM; 0.1–500μM and 5.0–500μM with detection limits of 0.009μM, 0.05μM and 1.0μM respectively using square wave voltammetric method. The modified electrode has been applied for the selective and precise analysis of 6-mercaptopurine, 6-thioguanine and dasatinib in pharmaceutical formulations and urine samples. •Simultaneous determination of 6-mercaptopruine, 6-thioguanine and dasatinib for the first time.•Synergistic effect of Pt/MWCNTs and 1-butyl-3-methylimidazolium hexafluorophosphate.•Real sample analysis of above compounds as anticancer drugs using novel sensor.