Development of an ELISA to detect clenbuterol in swine products using a new approach for hapten design

This research outlines the application of an enzyme-linked immunosorbent assay (ELISA) for the analysis of clenbuterol in animal products. Our assay showed good sensitivity for clenbuterol (0.4 ng/g or 0.4 ppb) and low detection limit (0.09 ng/g or 0.09 ppb). A low cross-reactivity for other β 2 -ag...

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Bibliographic Details
Published in:Analytical and bioanalytical chemistry 2016-09, Vol.408 (22), p.6045-6052
Main Authors: Bui, Quoc Anh, Vu, Thi Huynh Han, Ngo, Vo Ke Thanh, Kennedy, Ivan R., Lee, N. Alice, Allan, Robin
Format: Article
Language:English
Subjects:
Acids
Adrenergic beta-Agonists - analysis
Adrenergic beta-Agonists - immunology
Analytical Chemistry
Animal products
Animals
Antibody Formation
Assaying
Biochemistry
Characterization and Evaluation of Materials
Chemical properties
Chemistry
Chemistry and Materials Science
Chromatography
Clenbuterol
Clenbuterol - analysis
Clenbuterol - immunology
Composition
ELISA
Environmental monitoring
Enzyme-linked immunosorbent assay
Enzyme-Linked Immunosorbent Assay - methods
Enzymes
Epinephrine
Female
Food
Food Contamination - analysis
Food Science
Haptens - chemistry
Haptens - immunology
Identification and classification
Immunoanalysis for Environmental Monitoring and Human Health
Laboratory Medicine
Limit of Detection
Mass spectrometry
Mathematical analysis
Methods
Monitoring/Environmental Analysis
Monitors
NMR
Nuclear magnetic resonance
Organic chemicals
Pork
Rabbits
Red Meat - analysis
Research Paper
Residues
Scientific imaging
Swine
Testing
Vietnam
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Summary:This research outlines the application of an enzyme-linked immunosorbent assay (ELISA) for the analysis of clenbuterol in animal products. Our assay showed good sensitivity for clenbuterol (0.4 ng/g or 0.4 ppb) and low detection limit (0.09 ng/g or 0.09 ppb). A low cross-reactivity for other β 2 -agonist drugs such as salbutamol, terbutaline, and epinephrine led to formatting an ELISA kit considered to have a high specificity for clenbuterol. A survey of Ho Chi Minh City pork market was conducted as part of the validation of our ELISA. ELISA results showed a surprisingly high value of contamination. However, it will be necessary to conduct a more statistically valid replicated survey with evaluation by other instrumental methods to obtain a definite conclusion. This ELISA kit will be used to monitor growth promoter residues in Vietnam’s animal products.
ISSN:1618-2642
1618-2650
DOI:10.1007/s00216-016-9750-2